. Laboratory work in bacteriology. Bacteriology. GELATIN AND POTATO MEDIA. 165 steam, or for a sterilizer, as is often the case, when the large Koch apparatus is employed. The autoclave.—As indicated heretofore, the spore, which is the most resistant form encountered, may resist the action of steam even for several hours. Steam escapes from the ordinary Koch sterilizer or its sev- eral modifications, at the ordinary at- mospheric pressure, and hence, the tem- perature never ex- ceeds 100°. If, how- ever, the steam is not allowed to escape, but remains confined in an appa- ratus, the tempera- t
. Laboratory work in bacteriology. Bacteriology. GELATIN AND POTATO MEDIA. 165 steam, or for a sterilizer, as is often the case, when the large Koch apparatus is employed. The autoclave.—As indicated heretofore, the spore, which is the most resistant form encountered, may resist the action of steam even for several hours. Steam escapes from the ordinary Koch sterilizer or its sev- eral modifications, at the ordinary at- mospheric pressure, and hence, the tem- perature never ex- ceeds 100°. If, how- ever, the steam is not allowed to escape, but remains confined in an appa- ratus, the tempera- ture of the steam will rise with the increase in pres- sure. In this way, steam having" a tem- perature of 110°, 120° or 130° can be readily obtained. The higher the tempera- ture of the steam, the more rapidly will the resistant spores be destroyed. Steam at 130°, under pressure, will destroy instantaneously, spores which are able to withstand 3 or' 4 hours' exposure at 100°. The Pasteur school recognized, at an early date, the value of steam under pressure as a means of effecting sterilization and, for that reason, in. Fig. 27. Autoclave of Lequeux (Wiesnegg).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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