. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. Fig. 2. Pseudo-replica of bone, etched with a 1 "o HNO3 Fig. 3 a and b. Pseudo-replicas of bone digested with trypsin, in b, a thin fragment of bone stripped oti" the replicated surface. which ossein has been removed (see fig. 1) follows the Wiener law on the mixed rod-shaped body (1,7, 16). According to Schmidt who made special obser- vations in this connection, such an optical behaviour is due to the penetration of fluids at diff"erent refrac- tion indices in the ul
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. Fig. 2. Pseudo-replica of bone, etched with a 1 "o HNO3 Fig. 3 a and b. Pseudo-replicas of bone digested with trypsin, in b, a thin fragment of bone stripped oti" the replicated surface. which ossein has been removed (see fig. 1) follows the Wiener law on the mixed rod-shaped body (1,7, 16). According to Schmidt who made special obser- vations in this connection, such an optical behaviour is due to the penetration of fluids at diff"erent refrac- tion indices in the ultramicroscopical holes already occupied by the micellae of osteomucoid and osteoal- buminoid. {b) A bone system resulting from inorganic parti- cles or crystallites dispersed in the ossein, according to Engstrom and Finean, could not subsist when ossein had been removed, as the particles would break down into an "incoherent" powder. On the contrary, bone from which ossein has been removed, subsists as a coherent structure though its resistance is greatly decreased. In addition, the polarizing microscope and the low- and wide-angle x-ray dif- fraction patterns demonstrate that the structural orientation of the inorganic fraction is unmodified. Finally the curve of form birefringence can be recorded from the bone without ossein (see above). (f) It is very important to note that the change observed in the low-angle scatter of the bone from which ossein has been removed according to Ga- briel's method appears as new evidence of the neces- sity to interpret the low-angle scatter of the bone as organic ellipsoidal particles enclosed in the holes circumscribed by the inorganic fraction. Indeed such a change is neither related to the orientation nor the physical state of the inorganic crystallites, both con- ditions being unmodified by Gabriel's treatment, according to the wide-angle x-ray diffraction pat- terns. Therefore it is more plausible to maintain that in the homogene
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