. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 500 1000 1500 Radius (inn) 2000 2500 01 Ol UH U _Q H a EL, 3 P. parvivipara. n=361. 500 1000 1500 Radius (urn) Figure 5. The relationship between the size of the intragonadal ju- veniles and the number of lube feet. (A) Paliriclla vivipara. r = (B) P parvivipara r = 10. R = radius. tional Diagnostics; Atlanta, Georgia), and mounted on slides. Optical sections of the specimens were made with a B1O-RAD MRC600 confocal microscope. Development of the skeleton was documented by uti- lizing the birefringent properties
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 500 1000 1500 Radius (inn) 2000 2500 01 Ol UH U _Q H a EL, 3 P. parvivipara. n=361. 500 1000 1500 Radius (urn) Figure 5. The relationship between the size of the intragonadal ju- veniles and the number of lube feet. (A) Paliriclla vivipara. r = (B) P parvivipara r = 10. R = radius. tional Diagnostics; Atlanta, Georgia), and mounted on slides. Optical sections of the specimens were made with a B1O-RAD MRC600 confocal microscope. Development of the skeleton was documented by uti- lizing the birefringent properties of the calcite ossicles. The larvae are opaque and whole mounts of early larvae fixed in glutaraldehyde, dehydrated in alcohol, and cleared in Histo-Clear were observed with polarized light. The sizes of the brachiolaria and newly metamor- phosed juveniles of P. vivipara and P. parvivipara were compared with these developmental stages in other Pa- tiriclla species. For P. regularis, P. e.\igita, and P. calcar, live larvae were measured with an ocular micrometer. For P. giinnii, measurements were made from pho- tographs of live larvae. Measurements of newly meta- morphosed P. exigiia were made from live specimens. Measurements of newly metamorphosed P. calcar and P. regularis were made from preserved specimens. Results Pre-metamorphic development Patiriclla vivipara and P. parvivipara have the same pattern of development. Except where noted, the follow- ing description applies to both species. The largest eggs encountered in the ovotestes of P. vi- vipara were pale orange primary oocytes and ova. These eggs had a mean diameter of 148 ^m (SE = , /; = 21). Ova were not encountered in the gonads of P. parvivi- para. For this species, the largest eggs seen were 135 ^m diameter (/; = 2). When the gonad was torn during dissection, minute gastrulae and brachiolaria larvae were released and swam in the seawater in the dissection dish (Fig. la, b). Due to the intragonadal location of
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology