. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^. Figure 5. (A) Light microscope phase contrast micrograph of the sperm suspension of Pelromyzon marinus used to prepare the sperm-specific nuclear proteins shown in Figure 3 and Figure 4. About 2-3% of the sample consisted oferythrocyte contamination (arrow). (B) Light microscope Feulgen stain of a section of an embedded sperm sample from Lampclrn nchardsani icslis. (C) Light microscope Feulgen stain of a sperm suspension from Branchiotoma fluruliu: The magnification was x 1000 in every case. of the sperm cXBranchiostomafl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^. Figure 5. (A) Light microscope phase contrast micrograph of the sperm suspension of Pelromyzon marinus used to prepare the sperm-specific nuclear proteins shown in Figure 3 and Figure 4. About 2-3% of the sample consisted oferythrocyte contamination (arrow). (B) Light microscope Feulgen stain of a section of an embedded sperm sample from Lampclrn nchardsani icslis. (C) Light microscope Feulgen stain of a sperm suspension from Branchiotoma fluruliu: The magnification was x 1000 in every case. of the sperm cXBranchiostomafloridae (Ccphalochordata) and Pctroniyion marinus (Agnatha), the analyses must be carried out in comparison to the different groups of deuterostomes for which the sperm-specific protein com- position is already well established. At present some in- formation is available about the chromosomal proteins of the sperm of echinoderms, ascidian tunicates, and fish (see also Fig. 7 and Table III). The sperm-specific proteins of the echinoderms have been extensively characterized. They consist basically of histone variants (type H of the classification outlined in the Introduction), although in most cases highly special- ized sperm-specific histone H1 or H2B fractions have been described (type H1) (see Fig. 7, lanes 1, 2, 3). Thus, within the Subphylum Asterozoa, several representative organ- isms of the Class Stelleroidea have been analyzed (see also Fig. 7, lane 1) (Subirana and Palau, 1968; Strickland ct til., 1980; Zalenskaya ct 1980). In the sperm of these organisms the histone HI fraction is different from its somatic counterpart, whereas the core histones (H2A, H2B, H3, H4) exhibit an electrophoretic mobility in PAGE that is indistinguishable from that of the somatic core histones (Zalenskaya ct a/.. 1980). Within the Sub- phylum Echinozoa, several organisms within each of the classes Echinoidea and Holothuroidea have been char- acterized (see Fig. 7, lane 2). In the Echinoi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
