. Carnegie Institution of Washington publication. BROWN ROT OF SOLANACEAE. I87 July 14.—Early Rose potatoes in the field (plants Nos. 1 to 120) were used for this experiment. The cultures used were 5 slant agar-tubes of July 1 r, from No. 4, March 1 (South Carolina); slime washed off into distilled water (150 ). The inoculations were made near the top of the plants with a hypodermic syringe, of the liquid being put into each plant. The stems were very tender and soft. Result.—First recorded signs on July 23, when 77 per cent showed signs, but they were slight for 9 days' incubation


. Carnegie Institution of Washington publication. BROWN ROT OF SOLANACEAE. I87 July 14.—Early Rose potatoes in the field (plants Nos. 1 to 120) were used for this experiment. The cultures used were 5 slant agar-tubes of July 1 r, from No. 4, March 1 (South Carolina); slime washed off into distilled water (150 ). The inoculations were made near the top of the plants with a hypodermic syringe, of the liquid being put into each plant. The stems were very tender and soft. Result.—First recorded signs on July 23, when 77 per cent showed signs, but they were slight for 9 days' incubation. July 16.—Burbank potatoes in the field (plants Nos. 121 to 240) were inoculated in the same manner as the above, using 6 slant agar-tubes of July 14, from No. 1, March 1; slime washed off into distilled water (150 ). Result.—On July 25, 76 per cent showed signs of disease. July 22.—Potatoes in the field (plants Nos. 241-480) were inoculated in the same manner as those of July 14. using 6 slant agar-tubes of July 17, descended from No. 1, March 1; slime washed off into distilled water (150 ). Result.—On July 27, 60 per cent showed signs of disease; almost no wilting. July 22.—Potatoes in the field, half Early Rose, half Burbank (plants Nos. 1 to 240), were inoculated from 6 slant agar- tubes of July 20, from No. 6, July 10 (descended from potato-stem from Norfolk, Virginia), employing the method of inoculation used on July 14. Result.—On July 27, 96 per cent of the Early Rose and 89 per cent of the Burbank showed signs of disease. July 22.—Early Rose and Burbank potatoes in the field—half of each variety—were inoculated (using plants Nos. 241- 480). The cultures were 6 slant agar-tubes, July 21. from slant agar of July 20 from Virginia. The bacteria were put on outside of stem with a hypodermic syringe, which was kept protected from the light; the needle was then passed through this liquid, and entirely through the stem, 10 pricks being made into ea


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