. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 460 M. A. KAPPER ET AL. or uptake was measured after 24 h for the 35, 45, and 100 \*.M spiked animals, and in the time intervals of 8-12 h and 20-24 h after the transfer for animals subjected to either 0, 175, or 350 \iM NH4C1. Foot tissue was sampled for NFS and FAA de- termination from 10%o acclimated snails (controls) and at h 12 and 24 after transfer from snails in the 0, 175, and 350 \iM NH4C1 spiked transfers. In both sets of salinity transfer experiments, a snail's activity was assigned a value of if its foot was
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 460 M. A. KAPPER ET AL. or uptake was measured after 24 h for the 35, 45, and 100 \*.M spiked animals, and in the time intervals of 8-12 h and 20-24 h after the transfer for animals subjected to either 0, 175, or 350 \iM NH4C1. Foot tissue was sampled for NFS and FAA de- termination from 10%o acclimated snails (controls) and at h 12 and 24 after transfer from snails in the 0, 175, and 350 \iM NH4C1 spiked transfers. In both sets of salinity transfer experiments, a snail's activity was assigned a value of if its foot was extended and attached to the substrate, if the foot was extended but not attached, and 0 if the foot remained withdrawn. Body water determination The amount of water in the soft tissues at each salinity was determined as the difference in the weight of the soft parts before and after lyophilyzation. Oglesby's (1975) beta value was calculated at each steady state salinity as an indicator of the degree of regulation of body water content. Statistical analyses The General Linear Model procedure and Duncan's Multiple Range option of the Statistical Analysis System (SAS Institute, 1982) were used in data analysis. A prob- ability level of was significant. RESULTS Steady state experiments Levels of ninhydrin-positive substances in foot tissue of Thais haemastoma were directly related to the acclimation salinity over the range of 5-35%o when expressed in terms of ^moles • g dry tissue weight"1 or /umoles • g tissue water"1 (Fig. 1). CO 550 500 450 400 350 300 250 200 150 100 50 X * SE + = pM-gdry'1 * - pM-g tissue water"1. I 10 15 20 25 30 35 30*10 10*30 FIGURE haemastoma SALINITY (%0) 1. Ninhydrin-positive substance concentrations (x ± , n = 12) in the foot tissue of Thais expressed as /umoles NPS • g dry tissue weight'1 (+) and as j/moles NPS • g body water"' (•).. Please note that these images are extracted from scanned page images that m
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
