. Bulletin of the Department of Agriculture. Agriculture; Agriculture. NOSEMA-DISEASE. 39 In the experiments it was found that Nosema apis was destroyed in all instances in which the temperature of the honey reached or exceeded 140° F. (60° C), a temperature at which the germ is killed by heat (p. 30). Sufficient data, therefore, have not been obtained to warrant a definite conclusion regarding the time required for the. Fig. 7.—Petri dish. The top half is slightly raised. Those used here are 4 inches in diameter. direct sunlight to destroy Nosema apis suspended in honey. The results obta
. Bulletin of the Department of Agriculture. Agriculture; Agriculture. NOSEMA-DISEASE. 39 In the experiments it was found that Nosema apis was destroyed in all instances in which the temperature of the honey reached or exceeded 140° F. (60° C), a temperature at which the germ is killed by heat (p. 30). Sufficient data, therefore, have not been obtained to warrant a definite conclusion regarding the time required for the. Fig. 7.—Petri dish. The top half is slightly raised. Those used here are 4 inches in diameter. direct sunlight to destroy Nosema apis suspended in honey. The results obtained from the experiments made in which aqueous sus- pensions were exposed to the sun give some idea as to the probable approximate time which would be required. PERIOD NOSEMA APIS REMAINS VIRULENT. PERIOD IN HONEY. In experiments made to determine the length of time Nosema apis remains virulent in honey a suspension of the parasite in honey was distributed in flasks, about one-haK pint being poured into each ; These were placed at room temperature and shielded from the light. After different intervals colonies were inoculated, the suspension from a single flask being used. The results obtained are included in Table XX. Table XX.—Period Nosema apis remains virulent in honey. Date of inoculation. Period in honey. Mbnihs. Days. 1 0 1 18 2 0 3 10 3 27 2 6 2 15 2 25 3 5 3 17 3 20 3 21 4 4 5 0 7 17 7 19 9 7 9 19 12 0 Results of inoculation. Oct. 20, Feb. 4, Feb. 24, Feb. 4, Jan. 16, July 14,1915,.. July 23, Oct. 21, June 11, Sept. 3, June 24, Oct. 21, July 24, Aug. 14, Mayl, June 9, Apr. 27, May 5, July 26, Nosema infection produced. Do. Do. Do. Do. No infection produced. Do. Do. Do. Do. Do. Do. Do. Do. Do. Do. Do. Do. Please note that these images are extracted from scanned page images that may have been digitally enhanced for
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