The Journal of laboratory and clinical medicine . es of kerosene that remain are removed by filtering,through Small folded filters, into the colorimeter chamber or another test solution should stand not less than five nor more than twenty minutes be-fore being compared in a Duboscq colorimeter with the standard described below. *From the Sheffield Lahoratory of Physiological Chemistry, Yale University. Estiuiatioii of Sugar in the Blood 805 Daylight from a north window is always used for reading the mirror being set at the angle to give the lightest yellow tone to the
The Journal of laboratory and clinical medicine . es of kerosene that remain are removed by filtering,through Small folded filters, into the colorimeter chamber or another test solution should stand not less than five nor more than twenty minutes be-fore being compared in a Duboscq colorimeter with the standard described below. *From the Sheffield Lahoratory of Physiological Chemistry, Yale University. Estiuiatioii of Sugar in the Blood 805 Daylight from a north window is always used for reading the mirror being set at the angle to give the lightest yellow tone to the testing out the method, this was found necessary in order to obtain uniformresults. If it is late in the day, the material, evaporated to drjmess, is left in theJena glass tube until the next morning. The standard color, with which the unknown solution is compared, is ob-tained from mgm. of dextrose, 5 of saturated picric acid, and 1 ofa 10 per cent solution of sodium carbonate, evaporated over a free flame, and I I I. RtADING OP COLORIMETER MILLIMETERS Fig. 1.—{.raph for ohtainiiig the percentage of dextrose in tlu- liloixi ( method). 806 The Journal of Laboratory and Clinical Medicine diluted to 10 as was the unknown. A satisfactory permanent standard con-sists of a solution of picramic acid, which has approximately the same color,and keeps perfectly. It requires gm. of picramic acid and gm. ofanhydrous sodium carbonate. The picramic acid -is dissolved, with the aid ofheat, in 25-30 of distilled water made alkaline with the sodium is cooled and diluted to one liter. It should be standardized against puredextrose.
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