. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 SHAPPIRIO, EICHENBAUM AND LOCKE activity decreases progressively, yielding a graph of a shape typical for acetyl- cholinesterases (AChE's) in vertebrate and invertebrate preparations (Augustins- son, 1949, 1903). In order to permit comparison between data obtained under conditions found optimal for assay of ChE in the present study, and the results of Van der Kloot's (1955) investigation, the substrate-activity curve for pupal brain ChE was also 40 30 o o o I 20 01 10. X -log (AThCh) FIGURE 2. ChE activity as a function
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 SHAPPIRIO, EICHENBAUM AND LOCKE activity decreases progressively, yielding a graph of a shape typical for acetyl- cholinesterases (AChE's) in vertebrate and invertebrate preparations (Augustins- son, 1949, 1903). In order to permit comparison between data obtained under conditions found optimal for assay of ChE in the present study, and the results of Van der Kloot's (1955) investigation, the substrate-activity curve for pupal brain ChE was also 40 30 o o o I 20 01 10. X -log (AThCh) FIGURE 2. ChE activity as a function of AThCh concentration. The solid line describes enzymatic activity, which was corrected for spontaneous hydrolysis of AThCh at substrate con- centrations above 10"* M. The broken line describes the rate of spontaneous hydrolysis of AThCh. To provide sufficient material for the experiment illustrated, brains of six unchilled Cecropia pupae were pooled before homogenization. Enzyme activity is shown in relative units: one unit corresponds to a change in absorbance of per minute. determined under our "routine" assay conditions, but supplemented by sodium chloride to yield a final concentration of M. The latter had been employed in Van der Kloot's experiments owing to the findings of Chadwick ct al. (1953) show- ing that it yielded optimal ChE activity in homogenates of brains from flies. Our experiments with Cecropia showed that the substrate-activity curves with and without extra salt possess similar shapes. However, the addition of salt alters. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Red
Size: 1514px × 1651px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
