. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 54 APPLIED BACTERIOLOGY through muslin, after which 5 grammes of sodium chloride and 10 grammes of peptone are added; the mixture is then boiled for five minutes. The lic[uid is now carefully neutralised with a solution of sodium carbonate, and then rendered just faintly alkaline. Should the broth have been made too alkaline, a little dilute lactic acid is added until the proper degree ,of alkalinity is reached. The broth is now poured into a large flask
. Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians. Bacteriology. 54 APPLIED BACTERIOLOGY through muslin, after which 5 grammes of sodium chloride and 10 grammes of peptone are added; the mixture is then boiled for five minutes. The lic[uid is now carefully neutralised with a solution of sodium carbonate, and then rendered just faintly alkaline. Should the broth have been made too alkaline, a little dilute lactic acid is added until the proper degree ,of alkalinity is reached. The broth is now poured into a large flask, to which has been added previously 100 to 120 grammes of the finest colourless leaf gelatine. The gelatine is allowed to soak for an hour or so,. Fig. 12.—Apparatus for Filtering Media. when the flask is immersed in a water-bath until the gela- tine is perfectly dissolved. The reaction of the mixture is again carefully tested with litmus, to see if it still remains just alkaline. Owing to the loss of water by evaporation, the volume of the media should be noted, and hot water added until the volume is about 1,100 The mixture is now cooled, and the white of one egg is carefully and thoroughly mixed in with the gelatine solution, which is then heated in a boiling-water bath for fifteen minutes, or until the whole of the albumin has been precipitated. The. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Pearmain, Thomas Hames; Moor, Cresacre George. London, Baillie?re, Tindall and Cox
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectbacteri, bookyear1898
