. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. 1 ' \ \ \ I '/ion MM Fig, 'c^ r\C| < fiaoMM Fig. 48. t at 28° C. On agar streaks it sporulated freely after some days, the protoplasm, rounding off into two or more parts. Often three spore bodies and sometimes four or more in a cell were observed (fig. 49). The appearance and behavior of this yeast sug- gested Saccharoniyces cerevisiae. Sterile litmus milk inoculated by putting some of the grains into it did not redden, did not coagulate at once and did not produce any gas- bubbles. Gradually the litmus was


. Bacteria in relation to plant diseases. Bacteriology; Plant diseases. 1 ' \ \ \ I '/ion MM Fig, 'c^ r\C| < fiaoMM Fig. 48. t at 28° C. On agar streaks it sporulated freely after some days, the protoplasm, rounding off into two or more parts. Often three spore bodies and sometimes four or more in a cell were observed (fig. 49). The appearance and behavior of this yeast sug- gested Saccharoniyces cerevisiae. Sterile litmus milk inoculated by putting some of the grains into it did not redden, did not coagulate at once and did not produce any gas- bubbles. Gradually the litmus was reduced and remained reduced for a very long time. The milk also finally curdled. It was blue after the reduction ceased. It would appear, therefore, that some kefir-like grains contain a yeast incapable of fermenting lactose, while grains from other sources contain a yeast capable of fermenting lactose. The relation of kefir to the ginger-beer fermentation remains to be Fig. 49.; *FiG. 46.—Free-hand unstained section through one of the "beer-seeds," surface of grain being at left. The uniform gray structure represents bacterial zoogloeae, the darker masses the undisturbed position of imprisoned clumps of yeast. fFlG. 47.—A small portion of "beer-seed" crushed in water and drawn after staining with carbol fuchsin. The yeasts buried in the zoogloeae were not budding, most of them were globose or nearly so. JFiG. 48.—Another crushed-out water mount of "beer-seed," showing both round and elongate yeasts, the con- tents omitted, x and y stained alike with fuchsin and both forms were full of coarse granules. §FiG. 49.—Spores or spore-like bodies in yeasts on agar sub-culture (streak) made from an agar-plate poured from crushed "; Age 16 days. Stain, carbol Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appear


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