. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DKiKSTlVK KNZYMICS OF DAPHNIA 211 Baker and llulton (1920) method for the estimation of sugars and used as an indication of amylase activity. A 10-cc. sample starch solu- tion, pH , was diluted to 50 cc. and used as the substrate. To this was added 1 cc. of extract and thymol for preservative, and it was then kept at 37° C. Every 15 minutes 5-cc. samples were withdrawn and titrated. The increase in the amount of thiosulphate used in the titra- tion was equivalent to the same amount of maltose liberated in the digest. Dupl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DKiKSTlVK KNZYMICS OF DAPHNIA 211 Baker and llulton (1920) method for the estimation of sugars and used as an indication of amylase activity. A 10-cc. sample starch solu- tion, pH , was diluted to 50 cc. and used as the substrate. To this was added 1 cc. of extract and thymol for preservative, and it was then kept at 37° C. Every 15 minutes 5-cc. samples were withdrawn and titrated. The increase in the amount of thiosulphate used in the titra- tion was equivalent to the same amount of maltose liberated in the digest. Duplicate samples were used. The curves in Fig. 3 represent the mean of two runs. The control showed no hydrolysis during the 15 30 FIG. 3. Hydrolysis of starch at pH by amylase of hog pancreas (A), and of Daphnia (£?)• The ordinate represents the number of cc. of N/20 maltose; the abscissa is the time in minutes. The concentration of amylase is much greater in the glycerol extract of hog pancreas than in the Daphnia. After 30 minutes the hydrolysis of the starch by 1 per cent hog pancreas extract was equivalent to cc. of N/20 maltose, while in the same time cc. was the total amount produced by the Daphnia extract. LIPASE For the determination of lipase activity, 50 cc. of 4 per cent tribu- terine were emulsified with sodium glycocholate. To this were added 2 cc. of 1 per cent extract. The digest was placed at 37° C. and 10-cc. samples were withdrawn hourly and titrated with N/20 NaOH. The. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine
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