. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Tenebrionidae 465 later) after about 20 days. Pupae, if present, should be isolated by sexes into new and separate containers. This should be kept up until the desired number of pupae have been obtained from each bottle. 5. It is preferable at this point to select some of these isolated pupae and put them back into new stock bottles with fresh flour, thus completely renewing the stocks. It is important that the flour be changed, as Park (1934a, 1935) has shown
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Tenebrionidae 465 later) after about 20 days. Pupae, if present, should be isolated by sexes into new and separate containers. This should be kept up until the desired number of pupae have been obtained from each bottle. 5. It is preferable at this point to select some of these isolated pupae and put them back into new stock bottles with fresh flour, thus completely renewing the stocks. It is important that the flour be changed, as Park (1934a, 1935) has shown that old or "conditioned" flour reduces the fecundity and adversely affects the metamorphosis of the beetles. Flour infested with Tribolium may be sifted in such a way as to separate the beetles in all instars from it provided finely ground flour has been used. Chapman (1918, 1928) used standard meshes of silk bolting cloth and found that mesh number 9 would not pass any of the stages but would pass finely milled flour; number 3 segregated eggs and larger larval instars from the flour, and number 000 passed all eggs and. Fig. 81.—-Diagram of the automatic flour sifter. 1, belt wheel; 2, driving wheel; 3, driving rod; 4, flour sieve; 5, flour sieve holder; 6, removable collecting tray. (From Quart. Rev. Biol., with permission.) larvae except the largest instar. Thus, by using appropriate meshed sieves, all stages may be obtained as desired. To aid in this somewhat laborious sieving the author uses a mechanical device (Fig. 81) which automatically shakes the flour through the cloth and collects it in a re- movable tray below. The beetles are retained in the sieve. In handling the beetles it is well to be as gentle as possible; the author has found that both brush and small spatula are useful in this regard. In experimental work the adults should be removed from infected flour where possible before sifting since there is some evidence to the effect that too rough hand
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