. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. 144 BIOLOGY AND TECHNIQUE Plaitng.—The first method employed by Koch for bacterial isolations was one that consisted in the use of simple plates of glass, about 3x4 inches in size, mounted upon a leveling stand. A wooden triangle, supported upon three adjustable screw-feet, formed the base of this apparatus. Upon this was set a covered crystallizing dish which could be filled with ice water. Upon the top of this rested the sterilized plates under a bell j ar. By screwing up or down upo
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. 144 BIOLOGY AND TECHNIQUE Plaitng.—The first method employed by Koch for bacterial isolations was one that consisted in the use of simple plates of glass, about 3x4 inches in size, mounted upon a leveling stand. A wooden triangle, supported upon three adjustable screw-feet, formed the base of this apparatus. Upon this was set a covered crystallizing dish which could be filled with ice water. Upon the top of this rested the sterilized plates under a bell j ar. By screwing up or down upon the supports of the triangle, leveling of the plate could be achieved and controlled by a spirit- level placed at its side. The inoculated gelatin was poured upon the. Fig. 29.—Inoculating. plate and spread and rapidly cooled and hardened by the cold water contained in the crystallizing dish. The original method of Koch has been modified considerably and the method universally employed at present depends upon the use of circular covered dishes, the so-called Petri dishes. These obviate the necessity of a leveling stand and prevent contamination of the plate when once poured. Each Petri dish plate consists of two circular glass dishes; the smaller and bottom dish has an area of square centimeters; the larger is used as a cover for the smaller, and forms a loosely fitting Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Philip Hanson, 1868-1913; Zinsser, Hans, 1878-1940. joint author. New York and London, D. Appleton and Company
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