. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. 82 Bofanical Microtechnique When the surface of the celloidin is hard (2 niin.), press the twig gently until it is flat on the mounting block, thus affording firmer support for sectioning. A batch of embedded material usually con- tains more pieces than are needed for immediate sectioning, therefore, only a few pieces need to be mounted on blocks. Most of the pieces are merely removed from the thick celloidin, hardened in
. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. 82 Bofanical Microtechnique When the surface of the celloidin is hard (2 niin.), press the twig gently until it is flat on the mounting block, thus affording firmer support for sectioning. A batch of embedded material usually con- tains more pieces than are needed for immediate sectioning, therefore, only a few pieces need to be mounted on blocks. Most of the pieces are merely removed from the thick celloidin, hardened in chloroform, and stored in glycerin-alcohol. The pieces can be blocked at any future time bv the method to be described —Methods of mounliiig tissues on blocks for sectioning in celloidin: A, cutaway view of drilled block for holding long object; B and C, cutaway views of drilled bk)ck containing twig or other long object enveloped in hardened celloidin; D-F, infdlrated blocks of wood and bark embedded in hardened celloiciin. oriented on mounting blocks to cut transverse, radial, and tangential sedions. respectively. Sectors from large limbs usually must be fastened on mounting blocks for sectioning. II I he j)ieces ha\e cambium and other tissues of the bark, these tissues may peel off when the piece is compressed in the microtome clamp. Mount three pieces from each subject, on separate i), so that transverse, radial, and tangential sections can be cut (Fig. ^.2 D-f). A generous wrapping of celloidin should en- velop part of the mounting block as in Fig. The rigidity of the moiuuing of such material can be improved by mounting the material in a recess that has been drilled about l/Ki in. deep into the end of the mounting block. Blocking of pic'\ ioiisly hardened tinbttlded material is a simple operation. Remove the desired pieces fiom the glycerin-alcohol storage (liiid and soak in anhydrous ethyl alcohol, (-hange the alcohol t\\i(c a! 1- to S-hr. i
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Keywords: ., bookcentury1900, booksu, booksubjectbotany, booksubjectmicroscopy
