. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). paraquinoid formula OH NH. "N= orthoquinoid formula In the case of the paraquinoid formula the compound is an am- monium base of the type discussed on p. 15, which is capable of salt formation thru its pentavalent nitrogen. In the case of the orthoquinoid formula the salt formation takes place thru the tetravalent sulfur, the base being of the type known as a sulfonium base. There are arguments in favor of either formula, and from the standpoint o
. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). paraquinoid formula OH NH. "N= orthoquinoid formula In the case of the paraquinoid formula the compound is an am- monium base of the type discussed on p. 15, which is capable of salt formation thru its pentavalent nitrogen. In the case of the orthoquinoid formula the salt formation takes place thru the tetravalent sulfur, the base being of the type known as a sulfonium base. There are arguments in favor of either formula, and from the standpoint of the biologist it does not matter which is preferred. Possibly both forms actually exist simultaneously. For the sake of uniformity the paraquinoid form will be sho^-n in the following pages wherever possible; but with the understanding that the orthoquinoid form is equally permissable. The dye, thionin, is a salt, generally a chloride, of the above mentioned base; and on the assumption of paraquinoid structure, it has the following formula: /\ S _/\=XH, I CI "See Fig. 5. p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975; Ambler, Joseph Alfred, 1889-; Kornhauser, S. I. (Sidney Isaac), 1887-1959; Mallory, Frank Burr, 1862-1941; Sharp, Lester W. (Lester Whyland), b. 1887. Geneva, N. Y. , The Commission
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