. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 424 K. TURNER AND T. A. LYERLA. 123 4 5678 9 10 11 12 13 14 15 16 FIGURE 1. Polyacrylamide gel zymogram of amylase activity in Panopcus licrbstii. Chan- nels 1-10 are duplicate samples from five different P. licrbstii form "simpsoni" individuals, and channels 11-16 are duplicates from three different P. licrbstii form "obesa" individuals. Their inferred genotypes are as follows: channels 1-2 and 3-4, 109/105; channels 5-6, 113/113; channels 7-8, 103/103; channels 9-10, 105/105; channels 11-12, 125/105; ch
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 424 K. TURNER AND T. A. LYERLA. 123 4 5678 9 10 11 12 13 14 15 16 FIGURE 1. Polyacrylamide gel zymogram of amylase activity in Panopcus licrbstii. Chan- nels 1-10 are duplicate samples from five different P. licrbstii form "simpsoni" individuals, and channels 11-16 are duplicates from three different P. licrbstii form "obesa" individuals. Their inferred genotypes are as follows: channels 1-2 and 3-4, 109/105; channels 5-6, 113/113; channels 7-8, 103/103; channels 9-10, 105/105; channels 11-12, 125/105; channels 13-14, 125/103; channels 15-16, 105/105. At most, only two bands of amylase activity were resolved from a given individual, indicating a monomeric protein with electrophuretically separable allelic variants. and/or esterase zymogram patterns. Using Nei's index (Nei, 1975) to estimate genetic similarity, however, the two forms are genetically identical (I = ± SE, where I ranges from 1, or total identity, to 0, with no shared alleles in two populations). This is due to the large number of monomorphic loci at which the two forms shared electromorphs (20 out of 23). On an individual gene basis, those contributing to an identity of less than 1 were polymorphic: MDH-1 and AMY. Yet the two forms' dissimilar esterase zymogram patterns and the presence at the AMY locus of two alleles in "obesa" not found in "simpsoni" may indicate a greater genetic difference between these two populations than that implied by their designation as form variants (Rathbun, 1930). Differences between two populations of loci such as those coding for non-specific esterases may mean these could be considered as genetically divergent populations (Sarich, 1977). Although the two genera, P. hcrbstii and E. dcprcssus, were readily dis- tinguished at most loci, they shared electromorphs at EST-4, GOT, PRO-3 and MDH-2. Other similarities were also found. The scorable polymorph
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
