. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ANTARCTIC CEPHALOPOD HEMOCYANIN FUNCTION 69. X Gas mixture layer of mm Stirrer I Blood Figure 1. Cuvette used for the measurement of oxygen-binding prop- erties. Dashed areas show the compartments of the cuvette filled with blood. Absorbance was measured through a thin layer, thick, in the center part of the cuvette. Analysis of oxygen binding Oxygen-binding characteristics of cephalopod hemocya- nin were studied using a specially constructed cuvette, built by Hellma GmbH & Co. (Mulheim, Germany; Fig. 1). Th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ANTARCTIC CEPHALOPOD HEMOCYANIN FUNCTION 69. X Gas mixture layer of mm Stirrer I Blood Figure 1. Cuvette used for the measurement of oxygen-binding prop- erties. Dashed areas show the compartments of the cuvette filled with blood. Absorbance was measured through a thin layer, thick, in the center part of the cuvette. Analysis of oxygen binding Oxygen-binding characteristics of cephalopod hemocya- nin were studied using a specially constructed cuvette, built by Hellma GmbH & Co. (Mulheim, Germany; Fig. 1). The cuvette consisted of an upper and a lower compartment connected by two shafts ( and 2 mm in diameter) in the left and right periphery of the cuvette, as well as a central compartment between the shafts, where blood formed a thin layer of only mm. Stirring bars operating in the upper and lower compartments ensured continuous exchange of blood between all compartments and thus uniform mixture of the blood. Oxygen saturation was monitored continu- ously by using a diode array spectrophotometer with fiber optics (X-dap. IKS Optoelektronik MeGgeriite GmbH, Waldbronn, Germany) to measure absorbance at 345 nm through the thin layer. Blood samples were equilibrated by introducing humidified gas mixtures through a hole in the lid of the cuvette. Gas mixtures of variable Po, (between and kPa) were prepared from pure O,, CO,, and N, by gas-mixing pumps (type 2M303/a-F, Wosthoff, Bochum. Germany); complete deoxygenation occurred under pure N,. Blood pH was varied by changing Pco, (between and kPa) or by replacing small volumes (<10 ^\ per 2 ml of blood) of supernatant plasma after ultracentrifugation (1 h at X g; Beckman Airfuge, Beckman Instru- ments, Inc., Fullerton, CA) with fixed acid (1 mol 1~' HC1) or base (2 mol 1~' NaOH: Morris el ai, 1985; Portner, 1990). Changes in blood pH during oxygenation and deox- ygenation of hemocyanin were measured
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
