Wood's Medical and Surgical Monographs . te analysis 150 bacteria tothe cubic centimetre. This specimen kept constantly at 0°C. showed, twenty-four hours after, 520 bacteria to the cubcXII—28 420 Bacteriological Analysis of IValcr. centimetre. November 21st, that is four clays later, the num-ber of bacteria had increased to 1,750 to the cubic centimetre. The same sea water left twenty-four hours at an averagetemperature of 20° C. showed fabulous numbers of bacteria—many millions to the cubic centimetre. These facts being- established, I will be much more brief inmy description of practical mea
Wood's Medical and Surgical Monographs . te analysis 150 bacteria tothe cubic centimetre. This specimen kept constantly at 0°C. showed, twenty-four hours after, 520 bacteria to the cubcXII—28 420 Bacteriological Analysis of IValcr. centimetre. November 21st, that is four clays later, the num-ber of bacteria had increased to 1,750 to the cubic centimetre. The same sea water left twenty-four hours at an averagetemperature of 20° C. showed fabulous numbers of bacteria—many millions to the cubic centimetre. These facts being- established, I will be much more brief inmy description of practical means b^^ which water may beconveyed at a temperature approaching that of melting ice. For this purpose I employ the chest represented by Fig. 3,when the specimens are to be transported by rail. Th3 specimen of water furnished by the correspondent isput in the bottle F, with proper precautions, corked, and sealedup with sealing-wax; it is then wrapped in paper and placedwith slight friction in a metallic c^lnidrical box, where it re-. FiG. 3.—Refrigerator fur Lung Voyages. mains during the vo3age, sheltered from all shaking, conse-quently from all blows which might break it. This first box is placed in a second, larger in all its dimen-sions by some centimetres, and the emptj space is filled withsawdust. This system, well closed, is then placed in a muchlarger metallic box, which is filled with ice broken into largepieces. In summer, for journeys that last thirtj^-six hours (asI have assured myself by sending from those parts of Francemost remote from Paris), it requires six to nine pounds of ice. Finally, this third and last box, very firmlj^ closed, is buriedin sawdust in a wooden chest, B, furnished with a hinged topand a handle, P. The agent then delivers it without anj^ de-lay to the messengers, with orders to have it reach its destina-tion as quickly as possible. As a precautionary measure, the box should be sealed atits departure, and reach the analyst with the seal
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