. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY: CALCIUM REGULATION Reference: Biol. Bull 187: 271-272. (October. 19941 Three-Dimensional Calibration of the Non-Invasive Ion Probe, NVP;, of Steady Ionic Currents Joseph G. Kunkel and Peter J. S. Smith (Marine Biological Laboratory) 271 A non-invasive approach has been developed and applied to measure the flux of specific ions that enter and exit cells or tissues (1, 2). The NVP, adds, to the ion measurement technology of specific ion electrodes, an oscillation of the electrode over a short distance. V- differe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEUROBIOLOGY: CALCIUM REGULATION Reference: Biol. Bull 187: 271-272. (October. 19941 Three-Dimensional Calibration of the Non-Invasive Ion Probe, NVP;, of Steady Ionic Currents Joseph G. Kunkel and Peter J. S. Smith (Marine Biological Laboratory) 271 A non-invasive approach has been developed and applied to measure the flux of specific ions that enter and exit cells or tissues (1, 2). The NVP, adds, to the ion measurement technology of specific ion electrodes, an oscillation of the electrode over a short distance. V- differences with element O3U3 at the nominal point of closest approach to the diffusional source which has geometric location |x3, y0, z3} = {0,0,0} (Fig. 1). If one gradient estimate were made for each dimension at each grid position at Hz, the time to complete each 7X7 plane of data would be (3 X 7 X 7 X 3 s) = 7 min 21 s. The resultant planes of 49 dV0s can be in XZ plane, at y = -60 XT -6 I X-axiu (») Figure 1. Contour plot of 7X 7 observed ^ ^'-differences, d\ '„. at 20 urn internals in the plane of closest approach to a 100 mM Cad? source diffusing into a background concentration of 1 mM CaCl2. The point of closest approach was at O30i but the estimated axis of ion diffusion in this plane is 20 pm away at A, used to fit the unknown parameters of an expected /jV-difference equation. The probe need not be placed on the actual axis of diffusion but merely close to it. The position of the axis of dif- fusion. A0 = {XA. 0, ZA|. in theO-plane of closest approach { at y = 0) becomes another parameter to be fit by the equation of linear concentration relation with the inverse of distance, Cx, = Cb + K/(rxyl + !•„). The equation for the expected value of jjV-difference, dVE(r), to be measured at a radial distance, r, units along that concen- tration curve is now given by. dVE(r) = -103-S-eff-K-e' r-(Cb + K/(r + 6r/2)) where the effective radius from an observation
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