. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 566 D. T. LAMBERT AND M. FINGERMAN id O £4 t/) UJ a: S3 CL O O cc I u. 0 I 5 30 MINUTES 45 60 FIGURE 1. Effect of mersalyl acid on pigment concentration in leg melanophores of Uca pugilator. Opposite legs were removed; the experimental (dots) were perfused wth saline containing 37 HIM mersalyl acid, the controls (circles) with saline alone. Five pairs were used. Vertical lines representing one standard deviation on each side of the mean are included in this and the following graphs. among other reagents, glutathione, cystein
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 566 D. T. LAMBERT AND M. FINGERMAN id O £4 t/) UJ a: S3 CL O O cc I u. 0 I 5 30 MINUTES 45 60 FIGURE 1. Effect of mersalyl acid on pigment concentration in leg melanophores of Uca pugilator. Opposite legs were removed; the experimental (dots) were perfused wth saline containing 37 HIM mersalyl acid, the controls (circles) with saline alone. Five pairs were used. Vertical lines representing one standard deviation on each side of the mean are included in this and the following graphs. among other reagents, glutathione, cysteine hyclrochloride, and mersalyl acid. To further characterize the site of action of colchicine in crustacean chromatophores, these reagents \vere applied to the melanophores of Uca, using the isolated leg- perfusion technique. As has been described previously, perfusing legs with saline brings about pigment concentration; when high concentrations of colchicine are included in the saline, this pigment movement is inhibited (Lambert and Crowe, 1973). To determine whether glutathione or cysteine hydrochloride could prevent colchicine's inhibition of pigment concentration, as was reported for the melanophores of Fundulus (Wikswo and Novales, 1972b), these reagents were mixed with colchicine and perfused through isolated legs. The control legs were perfused with colchicine alone. At the three concentrations used (50, 100, and 155 mn), glutathione was unable to prevent inhibition by 15 HIM colchicine (data not shown). Similarly, 210 niM cysteine hydrochloride did not counteract inhibition by 21 mM colchicine (data not shown). In contrast to the differences between the melanophores of Uca and Fundulus described above, pigment concentration is inhibited in the melanophores of both upon treatment with mersalyl acid. As seen in Figure 1, perfusing mersalyl acid (37 HIM) through isolated legs greatly inhibits pigment concentration in the melanophores of these legs, as compared to the cont
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
