. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SCP-RFLMTD Pll'linrS IN CLAMS 431 Table I Durnhuiitiii of SCP-n'Ui/ctl nninii/imciklmn in ».vwti'.\ nl llic clam Mercenana mercenana Tissue p mole/g wet wt* Palps (480. 450) Visceral ganglia Cerebral ganglia Pedal ganglia Gills (178, 170) Rectum Intestinal typhlosole Mantle (1 13, 96) Foot (40, 30) Adducter muscles (23. 22. 60) Style sac typhlosole Heart 465 426 250 2(1(1 174 170 115t 105 35 35 9t 9 * Tissues from lOt or 20 animals were pooled, weighed, extracted in acetone, evaporated, and the aqueous portion fractionated b
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SCP-RFLMTD Pll'linrS IN CLAMS 431 Table I Durnhuiitiii of SCP-n'Ui/ctl nninii/imciklmn in ».vwti'.\ nl llic clam Mercenana mercenana Tissue p mole/g wet wt* Palps (480. 450) Visceral ganglia Cerebral ganglia Pedal ganglia Gills (178, 170) Rectum Intestinal typhlosole Mantle (1 13, 96) Foot (40, 30) Adducter muscles (23. 22. 60) Style sac typhlosole Heart 465 426 250 2(1(1 174 170 115t 105 35 35 9t 9 * Tissues from lOt or 20 animals were pooled, weighed, extracted in acetone, evaporated, and the aqueous portion fractionated by with analysis by RIA. The immunoreactivities of the peak fractions were added to produce the value indicated. Where more than one extract was tested, the means are reported in the right-hand column and the individual values are in parentheses. the peptides were expected to be similar to gastropod SCPs and to contain multiple methionine residues. After the acetone was removed and the extract filtered, the resulting clarified aqueous solution was loaded on a Prep 10 Aquapore Octyl column ( X 15 cm; Applied Biosystems) and eluted with an aqueous acetonitrile gra- dient containing trifluoroacetic acid (TFA; ' < through- out). The fractions were analyzed by radioimmunoassay (RIA; Price el ai. 1990). Each immunoreactive peak (consisting of one to three fractions) was further purified on a Spheri-5 RP18 ( X 220 mm), or an Aquapore Octyl RP300 ( X 220mm) column. In addition to aqueous acetonitrile gradients, we also used aqueous iso- propanol gradients, all containing TFA. In some cases, the fractions were treated with H2O2 (50 jul of 30%/ml) for 15 min to oxidize the methionyl residues of the peptides before re-chromatography (see Price el ai., 1990). In other cases, fractions were dried and incubated with dimethylsulfide (30 ^D- TFA (50 //I), and trifluoromethanesulfonic acid (TMFSA; 10 n\) for 3 h (basically following the Applied Biosystems "l
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