. dan bai zhi he mei xue yan jiu fang fa. botany. è³20X:,æ¶ä½å¨24å°æ¶å å½¢æã å®ä¾ï¼4ä¸â25«t:大è æè碱æ§ç£·é ¸é ¯é ¶ç»æ¶ã èç½è´¨æµåº¦20â25mg/mlï¼å å«56%饱ååº¦ç¡«é ¸æï¼, Tris-HCl ç¼å²æ¶²å 溶液温度éæ¸ä»4T:åå°25°Cã 6.é羼离å- å®ä¾ï¼ç¡«æ°§è¿èç½ï¼thioredoxin)ç»æ¶ã æ¬å¶åä»å°ï¼åç§ç»æ¶è¯éªåæªè·æåãå½èç½è´¨æµåº¦ä¸º5mg/mlæ¶ï¼ç¨å¾® ééææ³ï¼4<^ï¼å¯¹å¤é液ï¼ï¼ éé ¸é ç¼å²æ¶²å«éé ¸éï¼20 â .25%ä¹éï¼éæ2天åï¼æ¶ä½åºç°ã å èæç® [1 ] A. McPherson., Methods in Enzymology, H. W. Wyckoff, C. H. Hirs. and S. N. Timasheff, ed. Vol. 114, Academic Pre
. dan bai zhi he mei xue yan jiu fang fa. botany. è³20X:,æ¶ä½å¨24å°æ¶å å½¢æã å®ä¾ï¼4ä¸â25«t:大è æè碱æ§ç£·é ¸é ¯é ¶ç»æ¶ã èç½è´¨æµåº¦20â25mg/mlï¼å å«56%饱ååº¦ç¡«é ¸æï¼, Tris-HCl ç¼å²æ¶²å 溶液温度éæ¸ä»4T:åå°25°Cã 6.é羼离å- å®ä¾ï¼ç¡«æ°§è¿èç½ï¼thioredoxin)ç»æ¶ã æ¬å¶åä»å°ï¼åç§ç»æ¶è¯éªåæªè·æåãå½èç½è´¨æµåº¦ä¸º5mg/mlæ¶ï¼ç¨å¾® ééææ³ï¼4<^ï¼å¯¹å¤é液ï¼ï¼ éé ¸é ç¼å²æ¶²å«éé ¸éï¼20 â .25%ä¹éï¼éæ2天åï¼æ¶ä½åºç°ã å èæç® [1 ] A. McPherson., Methods in Enzymology, H. W. Wyckoff, C. H. Hirs. and S. N. Timasheff, ed. Vol. 114, Academic Press, New York (1985) 112. â f 2 ] J B. Sumner and G. F. Somers., "Chemistry and Methods of Enzymes" 2nd ed. Academic Press, New York (1947). [3 ] J. H. Northrop, M. Kuniiz and R. M. Herriott., "Crystalline Enzymes" Columbia Univ. Press, New York (1948). [4] K- Bailey. Trans. Faraday Soc, 38. 186(1942). ⢠[5] T. B. Osborne., Am. Chem. J., 14, 662(1892). [6 ] R. K. Scopes., "Protein Purification", Springer-Verlag, New York (1982). [71 M. Dixon and E. C. Webb., "Enzymes", 3rd ed, Longman Group, London (1979). [8] A. McPherson, Jr., Methods of Biochemical analysis, Vol. 23 (1976), 272. [9 1 R. Czok and T. Bucher" Advance Protein Chemistry, 15, 315(1960). f 101 W. B. Jakoby., Aval. Biockcm., 26, 295(1968). Mcth( {11] R. Henderson., Nature, 287, 490( 1980). [12] C. W. Carter and C. W. Carter, Jr., J. Biol. Chcm. 1 Enzymology, Vol. 22, p. 248 (1971). ,12219(1979). liiiiiiiiiiiiiM黼Iè°éºµ. 22. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustratio
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