. The actinomycetes. Actinomycetales. CAUSATION OI' AM MA I, DISEASES 253 Hujwitl ohtaiiu'd I'lom limn;iii ;icl iiiomycosis pure cultures of the inijiciohic orn'Miiisni, the true causal i\(' aticul of the disease; its iii'owth on glycerol a^ar I'escniMed that of the tubercle hacilhis. In 1891, WoUT and Isiael reported on a comprehensive iu\-estij;ation of the morphol- ogy and jiathogenicity of the organism caus- ing actinomycosis. Tiiey considered the mi- croaerophilic actinomyees to he the only causative agent of tiie iuimaii and l)o\iiie forms of the distvise. It may l)e of interest to ([Uot
. The actinomycetes. Actinomycetales. CAUSATION OI' AM MA I, DISEASES 253 Hujwitl ohtaiiu'd I'lom limn;iii ;icl iiiomycosis pure cultures of the inijiciohic orn'Miiisni, the true causal i\(' aticul of the disease; its iii'owth on glycerol a^ar I'escniMed that of the tubercle hacilhis. In 1891, WoUT and Isiael reported on a comprehensive iu\-estij;ation of the morphol- ogy and jiathogenicity of the organism caus- ing actinomycosis. Tiiey considered the mi- croaerophilic actinomyees to he the only causative agent of tiie iuimaii and l)o\iiie forms of the distvise. It may l)e of interest to ([Uote h(M'(> the r(>sults on the cultix'ation of tlu> organism as reported by them ('I'r. l)y Wright): "It ^row l)est uiiiltM' aiia('n)l)ic coiKlitioiis and did not grow at room tem])eratvire. On llic surface of anaerobic agar slant cultures on the tliirtl, fourth and fifth da.\' numerous minute isolated dew-drop-like colonies appeared, the largest pin head in size. These gradually became larger and formed ball-like, irregularly rounded elevated nodules varying in size up to that of a millet seed, exceptionally attaining the size of a lentil or larger. As a rule the colonies did not become confluent, and an apparently homogeneous layer of growth was seen to be made up of separate nodules if examined with a lens. In some instances the colonies presented a prominent center with a lobulated margin and appeared as rosettes. A characteristic of the colonies was that they sent into the agar root-like projections. In aerobic agar slant cultures no growth or a slow and very feeble growth was obtained. In stab cultures the growth was sometimes limited to the lower portion of the line of inoculation or was more vigorous there. In bouillon, after three to five days, growth appeared as small white flakes, partly floating and partly collected at the bottom of the tube. Growth oc- curred in bouillon under aerobic conditions, but was better under anaerobic conditions. The micro- org
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