. Bulletin of the Department of Agriculture. Agriculture; Agriculture. RUST RESISTANCE OF OAT VARIETIES. About 15 seeds per pot were sown for the seedling work, and the plants were thinned later, so that on an average about 8 plants per pot were inoculated. Plantings were made every few days from November, 1915, to April, 1916. Thus, there was always a series of plants coming on; as soon as one series had been inoculated another was about ready. The cultures were kept in a cool greenhouse (night temperature, 50° to 55° F.; day temperature, 60° to 65° F.) and watered not less often than ev
. Bulletin of the Department of Agriculture. Agriculture; Agriculture. RUST RESISTANCE OF OAT VARIETIES. About 15 seeds per pot were sown for the seedling work, and the plants were thinned later, so that on an average about 8 plants per pot were inoculated. Plantings were made every few days from November, 1915, to April, 1916. Thus, there was always a series of plants coming on; as soon as one series had been inoculated another was about ready. The cultures were kept in a cool greenhouse (night temperature, 50° to 55° F.; day temperature, 60° to 65° F.) and watered not less often than every alternate day. The inoculations on the seedling. Fig. 1.—Glass-topped galvanized-iron moist chamber used for seedling plants. plants were always made when the first (seedling) leaf was still vigorous and of a normal green color; that is, when the plants were only 3 to 5 inches high. This first leaf was always the only one inoculated. All others, with the " shoot," were kept trimmed off. Spore material of both the oat rusts was obtained from the Min- nesota Agricultural Experiment Station and increased for use as needed on stock cultures of the White Tartarian oat, the variety used as a check. About ten varieties usually constituted the series treated on any one day, one set being inoculated with stem rust and the other with crown rust. No plants were left uninoculated, but one pot of White Tartarian serving as a check on the other varieties was always sown and inoculated with each series. The inoculations were made by removing urediniospores with a flattened needle from a leaf bearing a heavy infection and placing. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original United States. Dept. of Agriculture. [Washington, D. C. ?] : The Dept. : Supt. of Docs. , G. P. O.
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