. Bacteriology and the public health. Bacteriology; Public health. 466 APPENDIX sterilised brush, transfer the particulate matter which has collected on the candle into 10 of sterile water or broth. This is now a concen- tration or emulsion of the organismal content of the litre of water, and may be used for examination for special organisms. (a) B. enteritidis sporogenes.—Place 0-5 or 1 of the concentrated water in each of three tubes of 10-15 of fresh sterilised milk. It is important to use fresh milk, recently boiled, and cooled down before inoculation. After inoculation with
. Bacteriology and the public health. Bacteriology; Public health. 466 APPENDIX sterilised brush, transfer the particulate matter which has collected on the candle into 10 of sterile water or broth. This is now a concen- tration or emulsion of the organismal content of the litre of water, and may be used for examination for special organisms. (a) B. enteritidis sporogenes.—Place 0-5 or 1 of the concentrated water in each of three tubes of 10-15 of fresh sterilised milk. It is important to use fresh milk, recently boiled, and cooled down before inoculation. After inoculation with the water to be examined, put the three tubes into the water bath for fifteen minutes at 80° C, and after allowing them to cool, place them in a Buchner's tube or cylinder containing freshly-prepared pyro- gallic solution (pyrogallic acid, 120 grains, strong liquor potassae, 10 ). Accurately seal up the Buchner, and place it, containing the tubes, in the incubator at 37° C. The next day, or in forty-eight hours, examine for B. enteritidis sporogenes. If that organism is present, the following characteristic appearances—the enteritidis change—will be ap- parent (Klein). The cream of the milk will be torn and altogether dissociated by the development of gas, so that the surface of the medium becomes covered with stringy white masses of coagulated casein, enclosing a number of gas bubbles. The main portion of the tube formerly occu- pied by the milk will contain a colourless thin watery whey, with a few lumps of casein adhering here and there to the sides of the tube (see Plate 21, p. 307). If the tube be opened, there will be found to be an odour of butyric acid and an acid reaction. If some of the con- tents of the tube are stained, as slide preparations, the bacilli will be seen. (b) B. COli Communis (p. 46).—Take from 0*1 to 0*5 of the concentrated or sample water, and add to tubes of phenolated gelatine (-05 per cent, phenol), or litmus-lactose agar, and make pl
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