. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. -C I -I FIGURE 5. Immunoelectrophoreses of hemolymphs from Jasus lalendei. An agarose gel containing 1 5 ^l/cnr of spiny lobster antiplasma serum is used, (a) Crossed immunoelectrophoresis: from left to right plasmas with concentrated GME, diluted GME, and trypsin inhibitor; each antigen contains 300 lig of plasma proteins, (b) and (c) Line immunoelectrophoreses: (b) From left to right plasmas with NEM, PMSF H2O, and PMSF propanol. (c) From left to right samples of plasmas with GME and trypsin inhibitor. Antigens contain 600
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. -C I -I FIGURE 5. Immunoelectrophoreses of hemolymphs from Jasus lalendei. An agarose gel containing 1 5 ^l/cnr of spiny lobster antiplasma serum is used, (a) Crossed immunoelectrophoresis: from left to right plasmas with concentrated GME, diluted GME, and trypsin inhibitor; each antigen contains 300 lig of plasma proteins, (b) and (c) Line immunoelectrophoreses: (b) From left to right plasmas with NEM, PMSF H2O, and PMSF propanol. (c) From left to right samples of plasmas with GME and trypsin inhibitor. Antigens contain 600 Mg of proteins in each line. H shows the hemocyanin and C represents the clottable factor. The arrow shows a fraction cross-reacting with the fibrinogen-like factor. In supernatants separated from intact hemocytes (with saline solutions of NEM sodium citrate, PMSF, GME, or DC), one additional fraction appears when ex- periments are carried out for 48 h with a buffer at pH In Homarus this fraction has approximately 240,000 daltons. Since the fluorescence of DC conjugates then is partly located at this place, it is possible that this component represents either the true monomer of "fibrinogen" or a subunit of soluble fibrin. This result is in agreement with the earliest reports of Fuller and Doolittle (1971), who have shown that the clottable factor is built up from two subunits of about 200,000 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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