. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2B. Continuation of the recording from Figure 2A showing the terminal part of the extracardiac pulsation including ventilation of the left prothoracic spiracle. temperature. In this case, anemometric recordings were preceded by one or more days of continuous monitoring of respiratory dynamics using the isotonic transducer alone (at 25°C). This was necessary to recognize possible abnormalities in respiratory functions resulting from the attachment of spiracles to anemometric tubing. The ane- mometric technique permits
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2B. Continuation of the recording from Figure 2A showing the terminal part of the extracardiac pulsation including ventilation of the left prothoracic spiracle. temperature. In this case, anemometric recordings were preceded by one or more days of continuous monitoring of respiratory dynamics using the isotonic transducer alone (at 25°C). This was necessary to recognize possible abnormalities in respiratory functions resulting from the attachment of spiracles to anemometric tubing. The ane- mometric technique permits unrestrained movement of air across the spiracles. However, the records occasion- ally signaled suffocation or incomplete CO2 ventilation after prolonged anemometric recordings. Usually this was recognized by supernumerary, out-of-schedule pul- sations. In this case the connectors were dismantled for some time. In the majority of diapausing Cecropia pupae (15 specimens; some of them measured several times) there were regular bursts of CO2 release at 5 to 7 h inter- vals. In addition, there were brief expiratory outbursts of intratracheal gases associated with abdominal rotation once per 12-16 h. Figure 3 shows a typical sample of the combined ten- sio-anemometric recording during the interburst period. The lower trace from the isotonic transducer reveals rela- tive changes of internal body volume. It shows that the spiracles were hermetically sealed most of the time (inter- nal pressure was subatmospheric throughout). The clo- sure is indicated by the periods when the abdomen re- tracts due to decreasing pressure with a constant speed of 3 ^m per min. Volumetric calibration of this pupa under water revealed that 1 ^m of abdominal contraction was equivalent to 240 nl of internal volume. Thus, isotonic transducer can be used as a rapid and simple detector of O: consumption rate. The constant rate of 3 /urn • min~' of abdominal retraction in Figure 3 corresponded to O2 consumpt
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
