. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 366 R. T. HANLON ET AL. Figure 1. The culture tanks for Eupryinnti . (A) Each para- larval rearing chamber (1) held 30 squids and had a viewing port (2). Water flowed in through two tubes (3) in each chamber and exited ver- tically through the sand-covered sieve bottom, then flowed horizontally through mesh screens (4) to the drain (5). The white ruler is the stan- dard 12 inches. (B) The complete closed system and A-frame, showing the filter apparali i he location of the biological filter substrate (2) and the ch


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 366 R. T. HANLON ET AL. Figure 1. The culture tanks for Eupryinnti . (A) Each para- larval rearing chamber (1) held 30 squids and had a viewing port (2). Water flowed in through two tubes (3) in each chamber and exited ver- tically through the sand-covered sieve bottom, then flowed horizontally through mesh screens (4) to the drain (5). The white ruler is the stan- dard 12 inches. (B) The complete closed system and A-frame, showing the filter apparali i he location of the biological filter substrate (2) and the chambe: nning adults and for mating (3). with a 200-Mm-mesh screen bottom (Fig. 1). The cham- ber was immersed in a seawater tray tank to achieve shal- low water depths of only 3-6 cm. Water flowed into the top through rubber tubing and flowed out through the mesh bottom. The two inflow tubes were arranged along the edge to create a gentle circular water flow, which helped keep the hatchlings and prey items away from the sides. A thin layer of sand coated the mesh bottom. Two small seawater systems were used in the Marine Resources Center: one was a completely recirculating system of 340 1, and the other was an open system of 600 1. Local Woods Hole water was the original source, and this water was passed through a 1-^m filter and heated to about 23°C. The closed system consisted of an A-frame with a shallow tray tank for the rearing experi- ments above (70 cm X 78 cm X 9 cm deep) and a deeper tank below to house the biological filter. The biological filter was composed of crushed oyster shell with an un- dergravel filter; the oyster shell was 15 cm deep and spread over an area of 5250 cnr. The water was then pumped through a canister with a particulate filter and then through activated carbon and a UV filter. The flow rate was about 22 1/min, and about 30% of the water was replaced weekly. The open system was constructed sim- ilarly, with a top tray size of 76 cm X 130 cm X 9 c


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology