. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 302 HARUO KAXATAXI AXD HIKOKO SH1RAI. FIGURE 4. Spawning of ovarian fragment treated with magnesium-free sea water; (A) ovarian fragment before treatment with magnesium-free sea water; (B) same fragment discharging eggs in magnesium-free sea water. In order to determine whether or not the effect of calcium deficiency is rever- sible, ovarian fragments, ligated to prevent discharge of eggs, were kept in calcium- free sea water for 50 minutes and then placed in sea water for appropriate periods up to 90 minutes. When cut at th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 302 HARUO KAXATAXI AXD HIKOKO SH1RAI. FIGURE 4. Spawning of ovarian fragment treated with magnesium-free sea water; (A) ovarian fragment before treatment with magnesium-free sea water; (B) same fragment discharging eggs in magnesium-free sea water. In order to determine whether or not the effect of calcium deficiency is rever- sible, ovarian fragments, ligated to prevent discharge of eggs, were kept in calcium- free sea water for 50 minutes and then placed in sea water for appropriate periods up to 90 minutes. When cut at the ligated portion such fragments began to shed TABLE I Induction of spawning in ovarian fragments of Asterias amurensis when they were kept in calcium-free sea water for various periods and then transferred to sea water Duration of treatment with calcium-free sea water (minutes) Interval preceding discharge of eggs after transfer to sea water Degree of spawning 30 minutes after transfer to sea water 10 15 20 (no spawning) (no spawning) 4-5 minutes * ± 25 1-2 minutes + 30 4-5 seconds + 35 2-3 seconds + + 40 2-3 seconds + + 45 at once + + + 50 at once + + + 60 at once + + + -: no spawning; ±: very small amount of eggs; +: small amount of eggs; + + : inter- mediate amount of eggs; + + + : large amount of eggs (ovarian fragments practically empty).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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