. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMAGE INTENSIFIER RECORDING MACROFLASH STIMULUS ARTIFACT. IMAGE INTENSIFIER , 1 1 1 1 TV MONITOR INVERTED MICROSCOPE FIGURE 1. Experimental apparatus used to localize subcellular bioluminescent and fluorescent sources. A single cell from the holding tube was directed to the suction pipet without stimulating it. Bioluminescence resulting from mechanical stimuli delivered via the stimulator-controlled piezoceramic crystal or by the addition of acid to the seawater bath was recorded by a side window photomultiplier tube (PMT) v
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMAGE INTENSIFIER RECORDING MACROFLASH STIMULUS ARTIFACT. IMAGE INTENSIFIER , 1 1 1 1 TV MONITOR INVERTED MICROSCOPE FIGURE 1. Experimental apparatus used to localize subcellular bioluminescent and fluorescent sources. A single cell from the holding tube was directed to the suction pipet without stimulating it. Bioluminescence resulting from mechanical stimuli delivered via the stimulator-controlled piezoceramic crystal or by the addition of acid to the seawater bath was recorded by a side window photomultiplier tube (PMT) via a light guide situated in the bath. PMT output was displayed on a storage oscilloscope and the display was recorded on video tape. The image of the cell was focused on the photocathode of the image intensifier tube (IIT) via the inverted microscope. Video recordings of the IIT output phosphor provided spatial and temporal records of subcellular bioluminescent activity which could be simulta- neously displayed with the oscilloscope recordings of total light output. Photomicrographs of whole cells (as for Fig. 2) and Nomarski micrographs of cytoplasmic features were made with the internal camera before and/or after IIT recordings of bioluminescence and fluorescence. tation at the nm mercury line and also includes an FT 420 chromatic beam splitter with the associated barrier filter replaced by an interference filter (BP 520- 560) to eliminate the intense red chlorophyll fluorescence. Mechanical excitation was provided by a manipulator-held glass probe (tip diameter 50 /urn) driven by a piezoceramic bender (Gulton Industries) controlled by a Grass S44 pulse generator. Acid stimulation of bioluminescence was achieved either by local micropipet application of M acetic acid to the cell surface, or by addition of cc of 4 M acetic acid or 23 M or 6 M formic acid to the cc seawater bath. A fiber-optic light guide built into the chamber wall led to an EMI 9781A photo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
