. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. n 10 20 30 Incubation time (mm) PMA - catalase control 40 1 I I n LPS + 1 >Jg ml'1 catalase. Incubation time (min) Figure 4. Reduction of ferricytochrome c by amoebocytes from Actinia equina in vitro. Twenty-five microliters of washed cells in ocean salts (OS; final concentration x 10" ml"') was incubated with 25 M' of phorbol myristate acetate (PMA; final concentration 8 ^g ml ') (a) or lipopolysaccharide (LPS; final concentration 1 ^g ml"1) (b), 25 ^1 of OS and 50 ^1 of ferricytoc
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. n 10 20 30 Incubation time (mm) PMA - catalase control 40 1 I I n LPS + 1 >Jg ml'1 catalase. Incubation time (min) Figure 4. Reduction of ferricytochrome c by amoebocytes from Actinia equina in vitro. Twenty-five microliters of washed cells in ocean salts (OS; final concentration x 10" ml"') was incubated with 25 M' of phorbol myristate acetate (PMA; final concentration 8 ^g ml ') (a) or lipopolysaccharide (LPS; final concentration 1 ^g ml"1) (b), 25 ^1 of OS and 50 ^1 of ferricytochrome c (final concentration 64 ^M). For controls, PMA or LPS was replaced with 25 n\ of OS. An extra series of wells, in which 25 ^1 of catalase (final concentration 1 ^g ml ') was substituted for the OS, was also included for both PMA- and LPS- treated cells to enable the effect of catalase on ferricytochrome c reduction to be monitored over 30 min. The absorbance of each well was measured at 550 nm at 5-min intervals for 30 min. Values given are means ± SE; n = 5. An asterisk (*) indicates that differences between experimental and control values are significant at the 1% level. compared to the time zero controls. Our subsequent ex- periments to test for lysozyme-like activity also failed to reveal significant lysis of Micrococcus luteus cell walls at any of the ALS concentrations tested, indicating that lysozyme is not one of the main agents involved in bac- terial killing. Discussion The results of this study show that amoebocytes iso- lated from the mesenteric filaments of A. equina are ac- tively phagocytic in vitro and produce substances that are able to inhibit the growth of gram negative bacteria. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Mar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
