. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 ROOT AND IRVING power rather rapidly. By the time 50 per cent of the oxygen-combining power has been lost the pH of whole blood is about , whereas the pH of hemolyzed blood is as low as 6. The curves remain similar in shape, and the similarity sug- gests correspondence in the behavior of the hemoglobin inside and outside of the cell. The data of Figure 2, obtained by measurement of pH with the glass electrode, agree with the results shown in Figure 1. These curves cannot be exactly super- imposed on the corresponding
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 ROOT AND IRVING power rather rapidly. By the time 50 per cent of the oxygen-combining power has been lost the pH of whole blood is about , whereas the pH of hemolyzed blood is as low as 6. The curves remain similar in shape, and the similarity sug- gests correspondence in the behavior of the hemoglobin inside and outside of the cell. The data of Figure 2, obtained by measurement of pH with the glass electrode, agree with the results shown in Figure 1. These curves cannot be exactly super- imposed on the corresponding curves of Figure 1, since, as pointed out previously, the measured and calculated pH values disagreed by a constant. The data of Figure 3 show nearly the same effect on hemoglobin when the pH is modified bv lactic acid instead of carbon dioxide. This holds for both whole. FIGURE 3. Comparison of the relationship between oxygen-combining power of hemoglobin at 155 mm. Ch-pressure and pH in whole and hemolyzed blood of the tautog at 15° Centigrade when the pH (measured) is modified either by carbon dioxide or by lactic acid. and hemolyzed blood. There is no great difference in the effect of the acidity produced by the two acids on the oxygen-combining power of hemoglobin in whole blood. Hemolyzed blood is also affected according to pH, although at a lower pH than in whole blood. DISCUSSION The difference in the tension at which carbon dioxide begins seriously to affect the oxygen-combining power of hemolyzed tautog blood, as compared with the whole blood, led to the earlier conclusion (Root, Irving and Black, 1939) that hemolysis renders this blood insensitive to carbon dioxide. At that time, hemo- lyzed blood was treated with less than 100 millimeters carbon dioxide (maximum about 75 millimeters), and, as the present work indicates, there is no considerable loss in oxygen-combining power under these conditions. It can still be stated that hemolyzed blood is insensitive to moder
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
