. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SUBSTRATE-ENZYME ORIENTATION 263 Response of intact embryos and brei to sodium aside The same type of experiments as were carried out with NaCN were also per- formed with sodium azide at pH The concentration selected was M since higher concentrations up to and including M did not result in more pronounced effect. The respiratory rates of intact embryos and corresponding breis obtained in the presence of azide are given in Figure 5. The percentage inhibitions at the various developmental stages calculated on th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SUBSTRATE-ENZYME ORIENTATION 263 Response of intact embryos and brei to sodium aside The same type of experiments as were carried out with NaCN were also per- formed with sodium azide at pH The concentration selected was M since higher concentrations up to and including M did not result in more pronounced effect. The respiratory rates of intact embryos and corresponding breis obtained in the presence of azide are given in Figure 5. The percentage inhibitions at the various developmental stages calculated on the basis of averages from Figures 1 and 5 are shown in Figure 6. O O LJ QL D O I U o O o o o r?° O O O O o O. NoN3(.005M) o-WHOLE EGG 0-BREI 0_Q 3 Oo> * ~ 8 o o .00 0 00 10 20 1 ' 30 40 50 60 70 HOURS j 6 k 5 ( 1 10 5 1 1 1 2 1 |. 3 4 1 16 5 t 1 7 8 1 g STAGE FIGURE 5. Respiratory rates in the presence of azide of brcis and intact embryos during development. In general (Figs. 5 and 6) the behavior of intact embryos towards azide is not very different from that with cyanide. However the residual azide insensitive respiration is consistently greater than its cyanide sensitive counterpart throughout development. Furthermore, unlike the cyanide insensitive respiration which re- mains constant, the absolute value of the azide insensitive respiration rises during development. The actions of cyanide and azide on brei are strikingly different. Up until stage 13, M azide can decrease the respiration of brei by only 10 per cent. It should be emphasized that this relative ineffectiveness of azide as a respiratory inhibitor against brei is not confined to this concentration only. As may be seen from column 2 of Table III it is true for concentrations as high as Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perf
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