. Current herpetology. Reptiles; Herpetology. MATSUI ET AL.—TAXONOMY OF ENDANGERED SALAMANDER 27. Fig. 1. A map of northern Central Honshu, Japan, showing sampled localities of hynobiid species used in this study. For sample numbers, refer to text. Coarsely dotted area >1000 m asl. Finely dotted area >2000 m asl. from Yamagata Prefecture (sample 5, N=6) (Fig. 1). In the laboratory, liver samples were removed from fully anesthetized salamanders and stored at -84 C. Voucher specimens were fixed in 10% formalin, preserved in 70% ethanol, and deposited in the Graduate School of Human and Env
. Current herpetology. Reptiles; Herpetology. MATSUI ET AL.—TAXONOMY OF ENDANGERED SALAMANDER 27. Fig. 1. A map of northern Central Honshu, Japan, showing sampled localities of hynobiid species used in this study. For sample numbers, refer to text. Coarsely dotted area >1000 m asl. Finely dotted area >2000 m asl. from Yamagata Prefecture (sample 5, N=6) (Fig. 1). In the laboratory, liver samples were removed from fully anesthetized salamanders and stored at -84 C. Voucher specimens were fixed in 10% formalin, preserved in 70% ethanol, and deposited in the Graduate School of Human and Environmental Studies, Kyoto University (KUHE) (see Appendix). Supernate fraction and homogenates were examined by standard horizontal starch gel electrophoresis (Shaw and Prasad, 1970; Ayala et al., 1972), containing Starch Art (Starch Art Corp., Smithville, USA) and Connaught starch (Connaught Lab., Ontario, Canada) mixed in a 4:1 ratio and then suspended in buffer at a concentration of Enzymes exam- ined, locus designations, and buffer systems employed are listed in Table 1. Staining methods, genetic interpretations of allozyme data, enzyme nomenclature, E. C. numbers, abbreviations, and isozyme designations follow Nishikawa et al. (2001). We studied 19 enzyme systems coded by 24 presumptive loci (Table 1). Genetic vari- ability for each sample was assessed by the standard analyses: percentage loci polymor- phic (P, a locus is considered as polymor- phic unless the frequency of the most common allele exceeds ), the mean heterozygosity. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Nihon Hachu Ryoseirui Gakkai. Kyoto : The Herpetological Society of Japan
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