. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. characteristic of the colonies was that they sent into the agar root-like projections. In aerobic agar slant cultures no growth or a slowand very feeble growth was obtained. In stab cultures the growthwas sometimes limited to the lower portion of the line of inoculationor was more vigorous there. In bouillon, after three to five davs. MICRO-ORGANISMS BELONGING TO HIGHER BACTERIA. 461 growth appeared as small white flakes, partly floating and partlycollected at
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. characteristic of the colonies was that they sent into the agar root-like projections. In aerobic agar slant cultures no growth or a slowand very feeble growth was obtained. In stab cultures the growthwas sometimes limited to the lower portion of the line of inoculationor was more vigorous there. In bouillon, after three to five davs. MICRO-ORGANISMS BELONGING TO HIGHER BACTERIA. 461 growth appeared as small white flakes, partly floating and partlycollected at the bottom of the tube. Growth occurred in bouillonunder aerobic conditions, but was better under anaerobic organisms here grow in branching and interlacing filaments, w^hichlater tend to break into segments (see Fig. 148). The microorganismin smear preparations from agar cultures appeared chiefly as shorthomogeneous, usually straight, but also comma-like or bowed rods,whose length and breadth varied. In many cultures short plumprods predominated, and in others longer, thicker, or thinner individ- FlG. 148. Smear from bouillon culture of actinomyces. X 1500 diameters. (From Wright.) uals were more numerous. The ends of the rods often showedoval or ball-like swellings. Swollen clubs were formed irregularly inthe presence of blood or serous fluids. Some twenty guinea-pigs and rabbits were inoculated, most of themin the peritoneal cavity, with pieces of agar culture. Eighteen animalswere killed after four to seventeen weeks, and four were still alive sevento nine months after inoculation. Seventeen rabbits and one guinea-pig showed at the autopsy tumor growths mostly in the peritonealcavity and in one instance in the spleen. In the four animals stillliving tumors were to be felt in the abdominal wall. The tumorsin the peritoneal cavity were millet-seed to plum size, and weresituated partly on the abdominal wall and partly on the intestines,the omentum, the mesenter
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