. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PIGMENT-DISPERSING HORMONE 141 800 - N | 600 •I 400 0 1 200 N M n K n N 1 3 5 7 9 11 13 15 17 Cycle Number 19 FIGURE 5. Yields of amino acid residues during the sequence analysis of Cancer PDH ( nmol load) by stepwise Edman degradation, utilizing a gas-phase automated sequencer (Model 470 A, Applied Biosystems), coupled with HPLC identification of the resulting PTH-amino acids. Nomenclature of amino acids by the one-letter code: A (Ala), D (Asp), E (Glu), G (Gly), I (He), K (Lys), L (Leu), M (Met), N (Asn), P (Pro)', S (
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PIGMENT-DISPERSING HORMONE 141 800 - N | 600 •I 400 0 1 200 N M n K n N 1 3 5 7 9 11 13 15 17 Cycle Number 19 FIGURE 5. Yields of amino acid residues during the sequence analysis of Cancer PDH ( nmol load) by stepwise Edman degradation, utilizing a gas-phase automated sequencer (Model 470 A, Applied Biosystems), coupled with HPLC identification of the resulting PTH-amino acids. Nomenclature of amino acids by the one-letter code: A (Ala), D (Asp), E (Glu), G (Gly), I (He), K (Lys), L (Leu), M (Met), N (Asn), P (Pro)', S (Ser), V (Val). at about three column volumes (Fernlund, 1971). In extracts of eyestalks from Uca pugilator, 0-PDH is the major form of dispersing hormone (Rao et al, 1985). A peak corresponding to a-PDH is either not evident (Rao et al., 1985) or may account for 15 10 x o CL • Native O Synthetic. Picomoles/Crab FIGURE 6. Dose-related melanophore pigment-dispersing activity elicited by purified native PDH of Cancer magister and a synthetic preparation of this peptide (first called Uca PDH; Rao et al., 1985). Each data point (mean, standard deviation) was derived from 3 assays (5 destalked Uca per assay). The PDH activity is expressed as Standard Integrated Response values, as defined by Fingerman et al. (1967). Pantin's physiological saline (control) did not elicit any dispersion in the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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