. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 244 E. S. GUZMAN BARRON, F. GHIRETTI VOL. 12 (1953) TABLE VI INTERMEDIATES OF THE CITRIC ACID CYCLE FOUND IN THE CULTURE MEDIA OF CERTAIN MOULDS GROWN IN ACETATE AS THE MAIN SOURCE OF OXIDIZABLE MATERIAL The figures give micrograms per ml of culture media, blank subtracted. Aspergillus M. niveus M. tronellosus Citric acid 3-6 a-Ketoglutaric acid 3-6 Pyruvic acid* Malic acid 5-1 1-7 2-5 Succinic acid** + j- + Fumaric acid + + 1 -r
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 244 E. S. GUZMAN BARRON, F. GHIRETTI VOL. 12 (1953) TABLE VI INTERMEDIATES OF THE CITRIC ACID CYCLE FOUND IN THE CULTURE MEDIA OF CERTAIN MOULDS GROWN IN ACETATE AS THE MAIN SOURCE OF OXIDIZABLE MATERIAL The figures give micrograms per ml of culture media, blank subtracted. Aspergillus M. niveus M. tronellosus Citric acid 3-6 a-Ketoglutaric acid 3-6 Pyruvic acid* Malic acid 5-1 1-7 2-5 Succinic acid** + j- + Fumaric acid + + 1 -r Oxalic acid 73-0 Glycolic acid 76 18 * Determined as a-ketoglutarate Detected by paper chromatography, TABLE VII EFFECT OF MALONATE ON THE OXIDATION OF ACETATE BY MICRO-ORGANISMS Acetate, ; Malonate, Temp. 38°. Incubation time 90 minutes. All figures blank subtracted. Micro-organism PH O2 Uptake Control Malonate Baker's yeast, 51 mgs/vessel 4 0 1030 952 mgs 4 0 1524 407 51 mgs 4 0 40 Corynebacterium creatinovorans 5 54 770 100 Pseudomonas fluorescens 5 54 749 638 Merulius niveus 5 54 548 560 Tetrahymena geleii 5 54 471 475 Rhyzopus nigricans 5-54 249 250. Fig. 4. Oxidation of malonic acid by Aerobacler cero- genes. Buffer M phosphate pH Malonate 10 micromoles. Abscissa time in hours. Ordinate Oj up- take in Figures given are blank subtracted. Temp. °. Calculated O^ uptake for complete oxida- tion malonate 448 ; found (40% of total oxidation). References p. 249. 75%. Lynen, aware of the membrane impermeability of yeast, obtained malonate inhibition by disturbing the membrane on treatment of yeast cells with liquid nitrogen-^. At pH , the lowest pH for respiration of these microorganisms, acetate oxidation by C. creatinovorans was inhibited "^jyo', by Pseudomonas fluorescens, 15%; by Tetrahymena geleii, completel}'. The membrane of moulds seems more im- permeable to malonate: malonate
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