. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I "^ GLYCEROL. GLUCOSE ORIGIN PW- FIGURE 3. Radioautograph of chromatogram of 80% ethanol soluble extract of zooxanthellae treated as in Figure 2, showing labeled intracellular products. other was identified as glycerol-14C. No other labeled components were detected. Since most of the 14C released by freshly isolated zooxanthellae is glycerol, we conclude that some glycerol-14C is released in vivo by the zooxanthellae and then esterified with unlabeled animal compounds to form lipid. From 56 to 73% of the animal tissue-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I "^ GLYCEROL. GLUCOSE ORIGIN PW- FIGURE 3. Radioautograph of chromatogram of 80% ethanol soluble extract of zooxanthellae treated as in Figure 2, showing labeled intracellular products. other was identified as glycerol-14C. No other labeled components were detected. Since most of the 14C released by freshly isolated zooxanthellae is glycerol, we conclude that some glycerol-14C is released in vivo by the zooxanthellae and then esterified with unlabeled animal compounds to form lipid. From 56 to 73% of the animal tissue-14C was ethanol insoluble (I-lb). Radiochromatographic fingerprinting of an hydrolysate of this material revealed that nearly all of the 14C was ninhydrin-positive and referable to amino acids. Two compounds did not react with ninhydrin and behaved chromatographically like native and oxidized lipid, despite treatment of the insoluble fraction with hot alcohol. We conclude that the labelled ethanol-insoluble fraction of animal tissue is largely protein. (a) Quantitative analysis (1-3, J-3) Table V shows the percentage distribution of 34C in differentially soluble fractions of animal tissue and algae from three 24- hour reef incubations. In both tissues, the relative proportions of labeled cold TCA-soluble (amino acids, sugars, and other low molecular weight substances released by osmotic shock) and hot TCA-soluble (nucleic acids) fractions are roughly the same. It is the alcohol-soluble and alcohol-TCA-insoluble fractions which differ. In the animal tissue more than half of the activity is insoluble protein. About 20% is alcohol soluble. The reverse seems to hold for the algae,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
