. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Staining 763 In 200 cc. of water, 2 grams of lithium carbonate are dissolved and 2 grams of Merck's medicinal, Griibler's BX, or Koch's rectified methylene blue added. This mixture is heated moderately in a rice boiler until a rich polychrome has formed. To determine this a sample is examined in a test-tube every few minutes by holding it against an artificial light. As soon as a distinctly red color is obtained, the desired degree of heating has been re
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Staining 763 In 200 cc. of water, 2 grams of lithium carbonate are dissolved and 2 grams of Merck's medicinal, Griibler's BX, or Koch's rectified methylene blue added. This mixture is heated moderately in a rice boiler until a rich polychrome has formed. To determine this a sample is examined in a test-tube every few minutes by holding it against an artificial light. As soon as a distinctly red color is obtained, the desired degree of heating has been reached. After cooling it is filtered through cotton in a funnel. To one-half of this polychrome solution 5 per cent, of acetic acid is gradually added until a strip of litmus-paper shows above the line of demarcation a distinct acid reaction, when the remaining half of the solutionig added, so as to carry the reaction back to a: low degree of alkalinity. A weak eosin solution is now prepared, approximately per cent. French eosin, and this is added gradually while the mixture is being stirred until a filtered sam- ple shows the filtrate to be of a pale bluish color with a slight fluorescence. The mixture is allowed to stand for one day and then filtered. The precipitate which has separated is collected on a double piece of filter-paper and dried at room tem- perature (heating spoils it). When completely dried it can easily be removed from the paper and may then be dissolved without further washing in commercial 4. \'1 V V\^ ^\^U^;. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders Company
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License: Licensed
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
