. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 178 C. S. OGILVY ET AL. Roslral. Optic Lobe / Cerebellum Location of Spinal Cord Transection Left Vagus Right Vagus Figure 1. Dorsal view of bluefish brain, drawn from a photograph A eraniotomy exposed the cerebellum and upper cervical spinal cord. The placement of the spinal cord transection is indicated. When bilat- eral vagotomies were performed, the vagal fibers were transected at the same level as the spinal cord. at an angle of 30°. A recovery period of 30 min was al- lowed after the tilting episode. Blood pressure and
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 178 C. S. OGILVY ET AL. Roslral. Optic Lobe / Cerebellum Location of Spinal Cord Transection Left Vagus Right Vagus Figure 1. Dorsal view of bluefish brain, drawn from a photograph A eraniotomy exposed the cerebellum and upper cervical spinal cord. The placement of the spinal cord transection is indicated. When bilat- eral vagotomies were performed, the vagal fibers were transected at the same level as the spinal cord. at an angle of 30°. A recovery period of 30 min was al- lowed after the tilting episode. Blood pressure and heart rate measurements were made from the polygraph rec- ord at regular intervals throughout the experiment. To interrupt central descending sympathetic pathways and produce muscular flaccidity, we transected the spi- nal cord after placing the ventral aortic catheter as de- scribed above. To create spinal cord lesions, we removed the skin and musculature over the posterior one third of the skull. This exposed the central spine of the skull which was removed using an electric drill with a burr at- tachment (Dremel Moto-Tool, model 370-5, Racine, Wisconsin). A eraniotomy was then performed using the burr attachment to carefully remove bone and expose the dura overlying the upper cervical region and lower medulla. The dura was then opened sharply and the spi- nal cord was exposed (Fig. 1). The vagal nerves run adja- cent to the spinal cord at the level of our dissection and these fibers were carefully separated from the spinal cord using microsurgical instruments and magnification. Af- ter vagal fibers were clearly separated, the spinal cord was sharply transected using microscissors at a level 3 mm caudal to the obex (Fig. 1). Following spinal cord transec- tion, the fish was left undisturbed for 20 min while blood pressure and heart rate were continuously monitored. The fish was then tilted head-up to 30° for 30 min. After returning to the horizontal position, blood pressur
Size: 998px × 2503px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
