. The elements of pathological histology with special reference to practical methods . ransparent. In order to test its sterility,the tubes of serum are kept for several days at incubation tem-perature, and those are removed in which bacteria or moulds havedeveloped during that time. Instead of the serum of animals, that obtained from the bloodof the human placenta, or serous fluid from tiie cavities of the humanbody, may be used. (d) Potatoes.—Potatoes of a moist kind are chosen, freed fromadherent clay by brushing with water, and left for half an hour in 38 BACTERIOLOGICAL METHODS 0*1 per ce
. The elements of pathological histology with special reference to practical methods . ransparent. In order to test its sterility,the tubes of serum are kept for several days at incubation tem-perature, and those are removed in which bacteria or moulds havedeveloped during that time. Instead of the serum of animals, that obtained from the bloodof the human placenta, or serous fluid from tiie cavities of the humanbody, may be used. (d) Potatoes.—Potatoes of a moist kind are chosen, freed fromadherent clay by brushing with water, and left for half an hour in 38 BACTERIOLOGICAL METHODS 0*1 per cent, corrosive sublimate solution. They are then cooked inthe steam apparatus, in a special holder (Fig. 6, d), for about threequarters of an hour, held fast with the thumb and one or two fingersof the left hand (dipped in sublimate solution) and cut in halves withbroad-bladed knives sterilised by lieat, and the halves are then kept inmoist chambers. The latter are prepared by covering the bottom of aglass dish with moist blotting-paper, and inverting a bell-glass overit (Fig. 7).. Fig. 7.—Moist Chambkr, with glass benches and culture plates. Another mode of preparing potatoes consists in peeling them whileraw, and then either cutting them into round slices or splittingthem obliquely into two halves. The former are placed in glassboxes of suitable size, the latter in test-tubes, and then sterilisedin the steam apparatus. Potatoes usually have a slightly acid reaction, and when it iswished for a special purpose to render them a feebly alkaline, thepieces, cut up raw, are deposited for five to ten minutes in a 5 or10 per cent, solution of soda, after which they are sterilised as usualby steam. {e) Bread pap.—Bread dried by a moderate heat is rubbed to afine powder, and 20 grm. of this having been introduced into an Erlen-meyers flask, enough water is added to convert it into a soft pap, whichis sterilised for an hour in the steam apparatus on three consecutivedays. On accoun
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectpatholo, bookyear1895
