. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ADENYLYL CYCLASE OF A DEEP-SEA FISH 69. B — — 120 TIME (min) Figure 2. Time course of R-PIA-induced dissociation of specific [3H]CHA binding in Antimora rostrata brain membranes. Membranes ( mg protein) were first incubated at 5°C with nM ['H]CHA for 240 min to allow binding to reach equilibrium. Sixty pM R-PIA was added in a negligible volume (1% of the total incubation volume) to initiate the dissociation reaction. The nonspecific binding, which has been subtracted from each experimental point, was det
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ADENYLYL CYCLASE OF A DEEP-SEA FISH 69. B — — 120 TIME (min) Figure 2. Time course of R-PIA-induced dissociation of specific [3H]CHA binding in Antimora rostrata brain membranes. Membranes ( mg protein) were first incubated at 5°C with nM ['H]CHA for 240 min to allow binding to reach equilibrium. Sixty pM R-PIA was added in a negligible volume (1% of the total incubation volume) to initiate the dissociation reaction. The nonspecific binding, which has been subtracted from each experimental point, was determined in the presence of 60 pM R-PIA. Inset depicts the first-order replot of dissocia- tion data and represents the best least-squares regression line. Data shown are from a single experiment. determined in the presence of 30 nAf R-PIA, saturated over the range of to approximately 16 nA/. Non- specific binding increased linearly as a function of ['HI- GH A concentration. Analysis of the [3H]CH A saturation isotherm, using equations based on mass action princi- ples, indicated that the data were adequately described by interaction with a single high affinity state of the re- ceptor. Computer modeling of replicate experiments. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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