. Fungous diseases of plants, with chapters on physiology, culture methods and technique . GERMINATION STUDIES 59. Fig. 13. Rings for Drop Culturesa, cemented to slide ; b, in Petri dish with filter paper possible. It is sometimes desirable to distribute the spores pre-viously in a drop or small quantity of water, otherwise one mayget too many in the culture drop. The covers are then invertedover the glass ring and pressed down so as to leave only oneminute unsealed area. It is an unwise and an inaccurate plan to use in the bottom ofthe cell any other liquid than that used in the culture drop.
. Fungous diseases of plants, with chapters on physiology, culture methods and technique . GERMINATION STUDIES 59. Fig. 13. Rings for Drop Culturesa, cemented to slide ; b, in Petri dish with filter paper possible. It is sometimes desirable to distribute the spores pre-viously in a drop or small quantity of water, otherwise one mayget too many in the culture drop. The covers are then invertedover the glass ring and pressed down so as to leave only oneminute unsealed area. It is an unwise and an inaccurate plan to use in the bottom ofthe cell any other liquid than that used in the culture drop. Thismust be so in order thatthere may be no differ-ences of vapor pressure,and consequently no evap-oration from drop to liquidbelow, or vice versa. Forinstance, it would be man-ifestly absurd to test ger-mination in a drop of, say,5 per cent alcohol above ifthere were only pure water below. If there is danger of contami-nation below, and consequent interception of the light, thoroughsterilization must be given beforehand. If the drop cultures aremade as soon as the slides are prepared, sterilization should
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