. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COCKROACH NERVE SHEATH 281 1947), with increased paraffin content, proved an excellent embedding medium. Conventional staining techniques included: Mallory's triple stain, Masson's tri- chrome, Gomori's chromium-hematoxylin-phloxin (Gomori, 1941) and Holme's silver as modified by Batham and Pantin (1951). Desheathed specimens were fixed after physiological tests. A series of cords \vas stained by Laidlaw's method as described by Krnjevic (1954) after the fifth ganglion and one half of the connective between fifth and sixth g
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COCKROACH NERVE SHEATH 281 1947), with increased paraffin content, proved an excellent embedding medium. Conventional staining techniques included: Mallory's triple stain, Masson's tri- chrome, Gomori's chromium-hematoxylin-phloxin (Gomori, 1941) and Holme's silver as modified by Batham and Pantin (1951). Desheathed specimens were fixed after physiological tests. A series of cords \vas stained by Laidlaw's method as described by Krnjevic (1954) after the fifth ganglion and one half of the connective between fifth and sixth ganglia had been desheathed. The living cord was placed in silver nitrate and observed under strong illumination. One of this series was fixed, sec- tioned and counterstained with Mallory's (Fig. 3b).. FIGURE 3. (a) Normally sheathed last abdominal ganglion stained with Masson's trichrome. Note outer homogeneous layer and inner nucleated granular layer of sheath, (b) Normally sheathed last abdominal ganglion after silver nitrate treatment. Note adherence of inner and and outer sheath layers in region of shrinkage. Note silver granules in sheath, absence of gran- ules in interior of ganglion. Counterstained with Mallory's triple stain. OBSERVATIONS Osmotic changes The intact, normally sheathed cord was not observed to swell or shrink and nervous conduction was unaffected when total solute concentration was reduced to the equivalent of M NaCl by dilution or increased to M by adding NaCl or sucrose to normal Hoyle's saline ( M). Desheathing in the hypotonic ( M) saline resulted in gross swelling and functional impairment. Synaptic conduction failed totally. Desheathing in the saline hypertonic to Hoyle's saline ( M) resulted in no immediate structural or functional Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations
Size: 1962px × 1273px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
