. Manual of bacteriology. Bacteriology. FILTRATION OF CULTURES. 17. FjG. 42. — Muencke's modiftcalion of Chamberland's filter. (c) Muencke's modification of the Chamberland principle is seen in Fig. 42. It consists of a thick-walled flask, a, the lower part conical, the upper cylindrical, with, a strong flange on the lip. There are two lateral tubes, ^ one horizontal to connect with exhaust-pipe, and one sloping, by which the contents may be poured out. Passing into the upper cylindrical part of the flask is a hollow porcelain cylin- der b, of less diameter than the cyhndrical part of flask a.
. Manual of bacteriology. Bacteriology. FILTRATION OF CULTURES. 17. FjG. 42. — Muencke's modiftcalion of Chamberland's filter. (c) Muencke's modification of the Chamberland principle is seen in Fig. 42. It consists of a thick-walled flask, a, the lower part conical, the upper cylindrical, with, a strong flange on the lip. There are two lateral tubes, ^ one horizontal to connect with exhaust-pipe, and one sloping, by which the contents may be poured out. Passing into the upper cylindrical part of the flask is a hollow porcelain cylin- der b, of less diameter than the cyhndrical part of flask a. It is closed below, open above, and rests by a projecting rim on the flange of the flask, an asbestos washer, c, being interposed. The fluid to be filtered is placed in the porcelain cylinder, and the whole top covered, as shown at f, with an india- rubber cap with a central perforation ; the tube d is connected with the exhaust-pump and the tube e plugged with a rubber stopper. When a large quantity of fluid is to be filtered, a receptacle such as that shown in Fig. 43 may be used. The tap in its bottom enables the filtrate to be removed without the apparatus being un- shipped, but it is difficult to get the tap to fit so accurately as not to allow air to pass into the vacuum chamber. Before any one of the above apparatus is used, it ought to be connected up as far as possible and sterilised in the Koch's steriliser. The ends of any important unconnected parts ought to have pieces of cotton wool tied over them. After use the bougie is to be sterilised in the autoclave, and —Flask after being dried is to be passed carefully through lain bougie for fii- a Bunscu flame, to burn off all organic matter. If tenng large quan- ^^ latter is allowed to accumulate, the pores become titles of fluid. ' ^ filled up. The success of filtration must be tested by inoculating tubes of media from the filtrate, and observing if growth takes place,. Please note that these images are ext
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