. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 30 MILTON FINGERMAX pigment dispersion or concentration alone was not supported by the data with extracts prepared from individual eyestalks. An experiment utilizing gel filtration on Sephadex LH-20 was then attempted to see if the dispersing and concentrating substances for the white pigment of Palaemonetes could be separated from each other by this technique. The averaged results of the experiment, performed three times, are shown in Figure 3. The white pigment-dispersing substance came off the column ahead of the white pi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 30 MILTON FINGERMAX pigment dispersion or concentration alone was not supported by the data with extracts prepared from individual eyestalks. An experiment utilizing gel filtration on Sephadex LH-20 was then attempted to see if the dispersing and concentrating substances for the white pigment of Palaemonetes could be separated from each other by this technique. The averaged results of the experiment, performed three times, are shown in Figure 3. The white pigment-dispersing substance came off the column ahead of the white pig- ment-concentrating substance, the former peaking in fraction 15 and the latter in fraction 20. 2 O 00 tr £ CO O •« a oo z o Sc Z LU O 2 O u II 15 19 FRACTION NUMBER 2 3 27 FIGURE 3. The white pigment-dispersing and -concentrating Standard Integrated Re- sponses (SIR) evoked by the fractions obtained by passing the ethanol-soluble material of the eyestalk through the column of Sephadex LH-20. Responses to extracts of the supraesophageal ganglia with the circumesophageal connectives attached The object of this group of experiments was to compare the responses to extracts of the supraesophageal ganglia with the circumesophageal connectives attached with the results presented above for the eyestalks. The first experiment consisted of a determination of the responses of extracts of freshly dissected supra- esophageal ganglia plus the circumesophageal connectives in isosmotic sea water. Extracts were prepared from groups of organ complements in a final concentra- tion of one-half organ equivalent (assayed on five prawns) and from single organ complements having a final concentration of one-fifth of an organ equivalent (assayed on three prawns). The experiment was performed four times, always with the same result. Each extract produced only dispersion of the white pigment. The averaged results of the four experiments are presented in Figure 4. The. Please n
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