. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 374 K. INOUE ET AL M1 2345678 9101112M. 190- 147- 111- Figure 6. Amplification of the variable region of the adhesive protein gene of wild and cultured mussels. Amplified products were electropho- resed on NuSieve GTG agarose gel (FMC). Lanes I-3, wild mussels collected at Tromso; Lanes 4-6, mussels cultured in Bnttans; Lanes 7- 9. wild mussels collected at Hiura: Lanes 10-12. mussels cultured on the Mediterranean coast of France. M. molecular marker (pUC19 DNA digested with Hapll). exhibited two fragments are presumed
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 374 K. INOUE ET AL M1 2345678 9101112M. 190- 147- 111- Figure 6. Amplification of the variable region of the adhesive protein gene of wild and cultured mussels. Amplified products were electropho- resed on NuSieve GTG agarose gel (FMC). Lanes I-3, wild mussels collected at Tromso; Lanes 4-6, mussels cultured in Bnttans; Lanes 7- 9. wild mussels collected at Hiura: Lanes 10-12. mussels cultured on the Mediterranean coast of France. M. molecular marker (pUC19 DNA digested with Hapll). exhibited two fragments are presumed to be hybrids between the two species. Discussion Among the five species of the genus Mytilus. M. edulis. M. galloprovincialis, and M. trossulm have been called the "M. edulis complex. " Since they are morphologically similar and shell shape is often influenced by local envi- ronment, it is difficult to identify these species by mor- phological characteristics. Recently, allozyme characters have been used to clarify the taxonomy of these species (Koehn?/a/., 1984; McDonald and Koehn, 1988;Varvio etui, 1988; McDonald el al. 1991; Coustau el 1991: Viard et 1994). These characters are recognized as reliable markers, but data for multiple loci are required for accurate identification of all three species. Identifica- tion using mitochondria! DNA (mtDNA) sequences has also been described (Edwards and Skibinski, 1987; Blot ft 1990; Geller et 1993, 1994). Although such attempts were partially successful, it is still difficult to dif- ferentiate the three species unambiguously. In this study, we found that differences in a certain "variable region" of a sequence in the nonrepetitive domain of the foot protein 1 agree well with the taxonomic rank of species. It was also shown that the variations can be attributed to differences in the length of the fragments amplified by PCR. Thus the variable region may become an effective diagnostic marker. Beca
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