. A manual of bacteriology. Bacteriology. THE CULTIVATION OF BACTERIA. 93 In cases where a very deep stab-culture is made in'gelatin or agar, where the growth appears in the lower part of the tube by preference, it is supposed to be anaerobic. Koch covered part of the surface of a gelatin plate with a bit of sterihzed mica or a cover-glass; bacteria which grew beneath tliis place were considered to be anaerobic. Another method was to cover the suHace of the gelatin in the culture-tube with sterilized oil. W. H. Park has recommended a mixture of solid paraffin with 25 to 50 per cent, of fluid p
. A manual of bacteriology. Bacteriology. THE CULTIVATION OF BACTERIA. 93 In cases where a very deep stab-culture is made in'gelatin or agar, where the growth appears in the lower part of the tube by preference, it is supposed to be anaerobic. Koch covered part of the surface of a gelatin plate with a bit of sterihzed mica or a cover-glass; bacteria which grew beneath tliis place were considered to be anaerobic. Another method was to cover the suHace of the gelatin in the culture-tube with sterilized oil. W. H. Park has recommended a mixture of solid paraffin with 25 to 50 per cent, of fluid paraffin or albolene as a covering for the surface of anaerobic cultures. This mixture has a semi-. FiG. 31.—Streak culture of the potato bacillus (natural size), showing an aerobic organism which will not grow under a cover-glass. soHd consistency, and does nor retract at the edges on cooling. The paraffin prevents the absorption of oxygen except to a small extent at the edges. The method is useful for large quantities of culture material, as in flasks. Esmarch advised making roll-tubes, and after cooling them to fill them with a melted gelatin cooled down to near the point of solidification. Hueppe made use of eggs in their shells. The egg-shell was carefully cleaned, sterilized with a solu- tion of bichloride of mercury, washed with sterilized water and wiped dry with sterilized cotton. The end of the egg-shell was punctured with a hot needle. Through the opening thus made the inoculation was accomplished. The opening was closed with Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Williams, Herbert Upham, 1866-; Bolton, Benjamin Meade, 1857-1929. Philadelphia, P. Blakiston's Son & Co.
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