. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Control of Rate of Intracellular Respiration 73 synthesis of ATP in the respiratory chain. The residual synthesis of HMP is due to the fact that the first step of the a-ketoglutaric oxidase system is coupled with phosphorylation in a reaction which is not sensitive to DNP. With increasing concentrations of DNP, the rate of hydrogen transfer increases. 0 2x10-5 4x10-5 6x10-5 8x10-5 10-" Concn. of 2:4-dinitrophenol (m) Fig. 4. Effect of increasing concentrations of DNP on the rate of respiration of blowfly th
. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Control of Rate of Intracellular Respiration 73 synthesis of ATP in the respiratory chain. The residual synthesis of HMP is due to the fact that the first step of the a-ketoglutaric oxidase system is coupled with phosphorylation in a reaction which is not sensitive to DNP. With increasing concentrations of DNP, the rate of hydrogen transfer increases. 0 2x10-5 4x10-5 6x10-5 8x10-5 10-" Concn. of 2:4-dinitrophenol (m) Fig. 4. Effect of increasing concentrations of DNP on the rate of respiration of blowfly thoracic- muscle sarcosomes, and on the accompanying esterification of inorganic phosphate. AP = esterified phosphate ((amoles), —AO = O2 uptake (jjiatoms), —AaKg = aketoglutarate oxidized ([xmoles). (From Slater and Lewis, 1954. Repro- duced by permission of the Editors, Biochem. J.) and consequently the amount of phosphorylation associated with the first step also increases, until eventually the increased phosphorylation in this step mor« than compensates for the complete inhibition of phosphorylation in the rest of the chain. The net result is that DNP increases the rate of phosphoryla- tion, although the efficiency of the conversion of oxidation energy to the synthesis of ATP is of course decreased. This experiment illustrates the feasibility of Lardy's. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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